重组
RabMAb

Anti-NRG3抗体[EPR4148] (ab109256)

概述

  • 产品名称
    Anti-NRG3抗体[EPR4148]
    参阅全部 NRG3 一抗
  • 描述
    兔单克隆抗体[EPR4148] to NRG3
  • 经测试应用
    适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the cytoplasmic domain of Human NRG3.

  • 阳性对照
    • Fetal brain, SH-SY5Y, C6, mouse brain, and rat brain lysates; Permeabilized C6 cells
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 77 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能
      Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
    • 组织特异性
      Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
    • 序列相似性
      Belongs to the neuregulin family.
      Contains 1 EGF-like domain.
    • 发展阶段
      Isoform 3 is expressed in fetal brain but not in other fetal tissues.
    • 结构域
      The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
      ERBB receptor binding is elicited entirely by the EGF-like domain.
    • 翻译后修饰
      Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
      Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
    • 细胞定位
      Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HRG3 antibody
      • Neuregulin 3 antibody
      • Neuregulin 3 like polypeptide antibody
      • Neuregulin-3 antibody
      • Neuregulin3 antibody
      • NRG-3 antibody
      • Nrg3 antibody
      • NRG3_HUMAN antibody
      • Pro neuregulin 3 antibody
      • pro NRG3 antibody
      • Pro-NRG3 antibody
      see all

    Anti-NRG3 antibody [EPR4148] 图像

    • All lanes : Anti-NRG3 antibody [EPR4148] (ab109256) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : C6 lysate
      Lane 4 : Mouse brain lysate
      Lane 5 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 77 kDa
      Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized C6 cells using ab109256 (red) or a rabbit IgG (negative) (green).

    Anti-NRG3 antibody [EPR4148] (ab109256)参考文献

    This product has been referenced in:
    • Liu Y  et al. KRASG12 mutant induces the release of the WSTF/NRG3 complex, and contributes to an oncogenic paracrine signaling pathway. Oncotarget 7:53153-53164 (2016). Read more (PubMed: 27449290) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (brain)
    Specification
    brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Tanveer Ahmed

    Verified customer

    提交于 Jul 15 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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