Anti-NRG3抗体(ab83704)

概述

  • 产品名称Anti-NRG3抗体
    参阅全部 NRG3 一抗
  • 描述
    兔多克隆抗体to NRG3
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region within the internal sequence 539-588 (TSINMQLPSRETNPYFNSLEQKDLVGYSSTRASSVPIIPSVGLEETCLQ M) of Human NRG3 (NP_001010848).

  • 阳性对照
    • Jurkat Cell Lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified by peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab83704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa.Can be blocked with NRG3 peptide (ab185897). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
  • 组织特异性Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
  • 序列相似性Belongs to the neuregulin family.
    Contains 1 EGF-like domain.
  • 发展阶段Isoform 3 is expressed in fetal brain but not in other fetal tissues.
  • 结构域The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
    ERBB receptor binding is elicited entirely by the EGF-like domain.
  • 翻译后修饰Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
    Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
  • 细胞定位Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HRG3 antibody
    • Neuregulin 3 antibody
    • Neuregulin 3 like polypeptide antibody
    • Neuregulin-3 antibody
    • Neuregulin3 antibody
    • NRG-3 antibody
    • Nrg3 antibody
    • NRG3_HUMAN antibody
    • Pro neuregulin 3 antibody
    • pro NRG3 antibody
    • Pro-NRG3 antibody
    see all

Anti-NRG3 antibody 图像

  • Anti-NRG3 antibody (ab83704) at 1 µg/ml (diluted in 5% skim milk / PBS buffer) + Jurkat Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cortex tissue labelling NRG3 with ab83704 at 4-8µg/ml.

Anti-NRG3 antibody (ab83704)参考文献

ab83704 has not yet been referenced specifically in any publications.

Product Wall

The NRG3 peptide has been added as item ab185897.


Please let me know if you have any questions, for instance regarding how to block the antibody with the peptide. In general, incubating equal amounts by weight of concentrated antibody and...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"