概述

  • 产品名称
  • 描述
    山羊多克隆抗体to NRG3
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    RNEIQRDSALTK

    , corresponding to amino acids 685-696 of Human NRG3 (NP_001010848.2)

  • 阳性对照
    • Human brain (cerebellum) lysate, mouse and rat brain lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/64000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 78 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
    • 组织特异性
      Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
    • 序列相似性
      Belongs to the neuregulin family.
      Contains 1 EGF-like domain.
    • 发展阶段
      Isoform 3 is expressed in fetal brain but not in other fetal tissues.
    • 结构域
      The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
      ERBB receptor binding is elicited entirely by the EGF-like domain.
    • 翻译后修饰
      Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
      Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
    • 细胞定位
      Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HRG3 antibody
      • Neuregulin 3 antibody
      • Neuregulin 3 like polypeptide antibody
      • Neuregulin-3 antibody
      • Neuregulin3 antibody
      • NRG-3 antibody
      • Nrg3 antibody
      • NRG3_HUMAN antibody
      • Pro neuregulin 3 antibody
      • pro NRG3 antibody
      • Pro-NRG3 antibody
      see all

    图片

    • Anti-NRG3 antibody (ab77597) at 0.3 µg/ml + human brain (cerebellum) lysate in RIPA buffer at 35 µg

      Predicted band size : 78 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    文献

    ab77597 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab77597.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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