Anti-NRG3抗体(ab77597)
Key features and details
- Goat polyclonal to NRG3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-NRG3抗体
参阅全部 NRG3 一抗 -
描述
山羊多克隆抗体to NRG3 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Cow, Dog, Chimpanzee -
免疫原
Synthetic peptide:
RNEIQRDSALTK
, corresponding to amino acids 685-696 of Human NRG3 (NP_001010848.2) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77597于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
说明 |
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WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 78 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes. -
组织特异性
Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes. -
序列相似性
Belongs to the neuregulin family.
Contains 1 EGF-like domain. -
发展阶段
Isoform 3 is expressed in fetal brain but not in other fetal tissues. -
结构域
The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
ERBB receptor binding is elicited entirely by the EGF-like domain. -
翻译后修饰
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated. -
细胞定位
Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form. - Information by UniProt
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数据库链接
- Entrez Gene: 10718 Human
- Omim: 605533 Human
- SwissProt: P56975 Human
- Unigene: 125119 Human
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别名
- HRG3 antibody
- Neuregulin 3 antibody
- Neuregulin 3 like polypeptide antibody
see all
图片
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Anti-NRG3 antibody (ab77597) at 0.3 µg/ml + human brain (cerebellum) lysate in RIPA buffer at 35 µg
Predicted band size: 78 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Note: The additional 37 kDa band of unknown identity was consistently observed and was successfully blocked by incubation with the immunizing peptide.
数据表及文件
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Datasheet download
文献 (1)
ab77597 被引用在 1 文献中.
- Paterson C et al. Temporal Dynamics of the Neuregulin-ErbB Network in the Murine Prefrontal Cortex across the Lifespan. Cereb Cortex 30:3325-3339 (2020). PubMed: 31897479