The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
功能Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. May also promote the heterodimerization with the EGF receptor.
组织特异性Restricted to the cerebellum in the adult.
序列相似性Belongs to the neuregulin family. Contains 1 EGF-like domain. Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
结构域The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization. ERBB receptor binding is elicited entirely by the EGF-like domain.
翻译后修饰Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. Extensive glycosylation precedes the proteolytic cleavage.
细胞定位Secreted and Cell membrane. Does not seem to be active.
Neural- and thymus-derived activator for ERBB kinases antibody
Neuregulin 2 antibody
Pro NRG2 antibody
Anti-NRG2 antibody 图像
Western blot - NRG2 antibody (ab56746)
Western blot against tagged recombinant protein immunogen using ab56746 NRG2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
Flow Cytometry-Anti-NRG2 antibody(ab56746)
Overlay histogram showing SH-SY5Y cells stained with ab56746 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56746, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Anti-NRG2 antibody (ab56746)参考文献
has not yet been referenced specifically in any publications.