概述

  • 产品名称Anti-NRG1 type III抗体
  • 描述
    兔多克隆抗体to NRG1 type III
  • 特异性ab23248 detects ~50-55kDa band in rat sciatic nerve, rat and mouse brain lysate. IHC detection with ab23248 in mouse DRG shows a primarily nuclear localisation of the protein rather than the expected cytoplasmic staining (See Bao and Wang references).
  • 经测试应用适用于: ICC/IF, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chicken, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human NRG1 type III.

    (Peptide available as ab23378.)

  • 阳性对照
    • mouse L4/5 dorsal root ganglion (DRG) tissue. Expected positive: mouse/rat sciatic nerve; neural crest derived neuroblasts

性能

应用

Our Abpromise guarantee covers the use of ab23248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 22366958
IHC-FoFr Use a concentration of 0.34 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa.

靶标

  • 相关性NRG1 type III is a sensory and motor neuron-derived factor isoform of Neuregulin 1, specifically expressed in the nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. NRG1 type III is the predominant isoform expressed in sensory and motor neurons. The Nrg1 gene encodes more than 15 transmembrane and secreted protein isoforms, generated by alternative promoter usage and mRNA splicing. Nrg1 subtypes I through III share the epidermal growth factor-like signaling domain and are defined by different amino-termini. The N-terminal cysteine-rich domain (CRD), as found in the sensory and motor neuronderived factor (SMDF), defines Nrg1 type III, which in embryonic development is responsible for survival of Schwann cell precursors.
  • 细胞定位Cell Membrane and Secreted
  • 数据库链接
  • 别名
    • Heregulin antibody
    • HRG antibody
    • HRG1 antibody
    • Neuregulin 1 antibody
    • NRG1 antibody
    • SMDF antibody
    see all

Anti-NRG1 type III antibody 图像

  • ab23248 detecting NRG1 Type III in mouse L4/5 dorsal root ganglion (DRG) tissue (perfused with 4% paraformaldehyde and cryoprotected overnight, cut on a cryostat at 15µm). Tissue was incubated in blocking serum (normal goat serum) for 1hr, washed 3XPBS before primary antibody incubation in PBS with triton (0.1%) overnight (ab23248 was used at 1/800 = 0.34µg/ml). Secondary antibody used was goat anti-rabbit Alexa 546 (1/1000). The image shown is taken with a X20 objective and shows mostly nuclear NRG1 Type III staining with some cytomplasmic staining (nuclear counterstain with DAPI confirmed this - not shown).

  • All lanes : Anti-NRG1 type III antibody (ab23248) at 1 µg/ml

    Lane 1 : Mouse Sciatic Nerve Lysate
    Lane 2 : Mouse Brain
    Lane 3 : Brain (Rat) Tissue Lysate (ab7942)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 27,52,74,75 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    ab23248 picks up an ~50kDa band in WB when tested on rat sciatic nerve, rat brain or mouse brain. This band size concurrs with an entry for NRG1 in both Expasy and NCBI  databases (Q3URY7). Carroll et al., (J Neurosci. 1997 Mar 1;17(5):1642-59) also detect a  50kDa band in dorsal root ganglion and spinal cord. However, it is surprising that we do not detect more bands; it is likely that ab23248 does not pick up all NRG1 type III isoforms or that there is very restricted expression in the tissues tested here.

Anti-NRG1 type III antibody (ab23248)参考文献

This product has been referenced in:
  • Yue Y  et al. Low-intensity pulsed ultrasound upregulates pro-myelination indicators of Schwann cells enhanced by co-culture with adipose-derived stem cells. Cell Prolif 49:720-728 (2016). Read more (PubMed: 27625295) »
  • Sango K  et al. Myelination in coculture of established neuronal and Schwann cell lines. Histochem Cell Biol 137:829-39 (2012). ICC/IF ; Rat . Read more (PubMed: 22366958) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kiney)
Permeabilization Yes - 0.1% troton x-100
Specification Kiney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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提交于 Jun 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 May 12 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.3% Triton x 100: 30 min
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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提交于 May 09 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 May 06 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (adult brain cerebellum)
Specification adult brain cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
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Mr. Carl Hobbs

Verified customer

提交于 Nov 09 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"