概述

  • 产品名称Anti-NRG1 type I抗体
  • 描述
    兔多克隆抗体to NRG1 type I
  • 经测试应用适用于: ICC/IF, IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human, Zebrafish
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human NRG1 type I.

    (Peptide available as ab32286.)

  • 阳性对照
    • WB: Human Brain Whole Tissue Lysate and Human Kidney Whole Tissue Lysate.
  • 常规说明


    ab27303 is NRG1 Type I specific and therefore is predicted to detect the NRG1 beta Type I isoforms 6-8 (NRG1 Beta1, Beta1A, Beta2, Beta3) in addition to the NRG1 alpha isoforms 1-4.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明ab27303 is NRG1 Type I specific and therefore is predicted to detect the NRG1 beta Type I isoforms 6-8 (NRG1 Beta1, Beta1A, Beta2, Beta3) in addition to the NRG1 alpha isoforms 1-4.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab27303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 70 kDa).
IP Use at an assay dependent concentration. PubMed: 21203579

靶标

  • 功能Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.
  • 组织特异性Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific.
  • 疾病相关Note=A chromosomal aberration involving NRG1 produces gamma-heregulin. Translocation t(8;11) with ODZ4. The translocation fuses the 5'-end of ODZ4 to NRG1 (isoform 8). The product of this translocation was first thought to be an alternatively spliced isoform. Gamma-heregulin is a soluble activating ligand for the ERBB2-ERBB3 receptor complex and acts as an autocrine growth factor in a specific breast cancer cell line (MDA-MB-175). Not detected in breast carcinoma samples, including ductal, lobular, medullary, and mucinous histological types, neither in other breast cancer cell lines.
  • 序列相似性Belongs to the neuregulin family.
    Contains 1 EGF-like domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 发展阶段Detectable at early embryonic ages. Isoform 10 is highly expressed in developing spinal motor neurons and in developing cranial nerve nuclei. Expression is maintained only in both adult motor neurons and dorsal root ganglion neurons. Type IV isoforms are expressed in fetal brain.
  • 结构域The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
    ERBB receptor binding is elicited entirely by the EGF-like domain.
  • 翻译后修饰Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
    N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage.
  • 细胞定位Secreted; Cell membrane. Does not seem to be active; Membrane. May possess an internal uncleaved signal sequence; Nucleus. May be nuclear and Secreted. Has a signal peptide.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acetylcholine receptor-inducing activity antibody
    • ARIA antibody
    • Breast cancer cell differentiation factor p45 antibody
    • GGF antibody
    • Glial growth factor antibody
    • Heregulin antibody
    • HRG antibody
    • Neu differentiation factor antibody
    • Neuregulin 1 antibody
    • Neuregulin-1 antibody
    • NRG 1 antibody
    • NRG alpha antibody
    • NRG beta antibody
    • nrg1 antibody
    • NRG1_HUMAN antibody
    • Pro-NRG1 antibody
    • Sensory and motor neuron-derived factor antibody
    • SMDF antibody
    see all

Anti-NRG1 type I antibody 图像

  • All lanes : Anti-NRG1 type I antibody (ab27303) at 1 µg/ml

    Lane 1 : Human Brain Whole Tissue Lysate
    Lane 2 : Human Kidney Whole Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 2 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution


    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)

    The predicted MW for Isoform 1 of this protein is ~70 kDa. The 51 kDa band observed in our data correlates with the predicted size of Isoform 3 (50.7 kDa). We believe the doublet is caused by the presence of a smaller processed form of Isoform 3.

    The epitope for ab27303 is present in all the described isoforms of the protein.

  • Immunohistochemistical (Formaldehyde/PFA-fixed paraffin-embedded sections) staining for NRG1 type I antibody (ab27303) on Zebrafish Tissue sections (Transverse section through whole body). This image shows specific staining for NRG1 Type 1 in the spinal cord. Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody ab27303 incubated at 1/100 for 16 hours at RT. Secondary Antibody: Biotin labelled goat anti Rabbit IgG (1/300).
  • ICC/IF image of ab27303 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab27303, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • IHC image of NRG1 type 1 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27303, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-NRG1 type I antibody (ab27303)参考文献

This product has been referenced in:
  • Marques-Smith A  et al. A Transient Translaminar GABAergic Interneuron Circuit Connects Thalamocortical Recipient Layers in Neonatal Somatosensory Cortex. Neuron 89:536-49 (2016). WB ; Mouse . Read more (PubMed: 26844833) »
  • Pan P & Dobrowsky RT Differential expression of neuregulin-1 isoforms and downregulation of erbin are associated with Erb B2 receptor activation in diabetic peripheral neuropathy. Acta Neuropathol Commun 1:39 (2013). Mouse . Read more (PubMed: 24252174) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Zebrafish Tissue sections (Transverse section through whole body)
Specification Transverse section through whole body
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 27 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Spinal cord rat d15 embryo)
Specification Spinal cord rat d15 embryo
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 22 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Paget's disease of the nipple)
Specification Paget's disease of the nipple
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 19 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Embryonic day 18 pancreas and small intestine)
Specification Embryonic day 18 pancreas and small intestine
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 12 2007

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Rat brain)
Specification Rat brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Sep 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"