RabMAb

Anti-Nrf2抗体[EP1808Y] (ab62352)

概述

  • 产品名称Anti-Nrf2抗体[EP1808Y]
    参阅全部 Nrf2 一抗
  • 描述
    兔单克隆抗体[EP1808Y] to Nrf2
  • 经测试应用适用于: ChIP, IHC-Fr, Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Nrf2 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab167152)

  • 阳性对照
    • HepG2 and HeLa cell lysate, human lung adenocarcinoma tissue FC: HeLa cells
  • 常规说明

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab62352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 22581777
IHC-Fr 1/100. Fix with acetone. Note: antigen retrieval is recommended.
Flow Cyt 1/40.
ICC/IF 1/100 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 68 kDa.Can be blocked with Nrf2 peptide (ab167152).
IP 1/20.
IHC-P 1/100 - 1/250.

靶标

  • 功能Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • 组织特异性Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • 序列相似性Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • 结构域Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • 翻译后修饰Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • 细胞定位Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • 数据库链接
  • 别名
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

Anti-Nrf2 antibody [EP1808Y] 图像

  • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling Nrf2 (green) with purified ab62352 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). DAPI was used to stain the nuclei blue.

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemical analysis of Nrf2 expression in paraffin embedded human lung adenocarcinoma using 1/100 ab62352
  • Anti-Nrf2 antibody [EP1808Y] (ab62352) at 1/2000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    goat anti rabbit HRP at 1/2000 dilution

    Predicted band size : 68 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Nrf2 with ab62352 at 1/40 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • ICC/IF image of ab62352 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62352, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Nrf2 antibody [EP1808Y] (ab62352)参考文献

This product has been referenced in:
  • Tian Y  et al. Modification of platinum sensitivity by KEAP1/NRF2 signals in non-small cell lung cancer. J Hematol Oncol 9:83 (2016). Read more (PubMed: 27601007) »
  • Spriggs S  et al. Effect of Repeated Daily Dosing with 2,4-Dinitrochlorobenzene on Glutathione Biosynthesis and Nrf2 Activation in Reconstructed Human Epidermis. Toxicol Sci 154:5-15 (2016). Read more (PubMed: 27492222) »

See all 31 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Sample Human Tissue sections (kidney cancer)
Specification kidney cancer
Permeabilization Yes - Wash Buffer with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 Aug 23 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Bone Marrow derived macrophages (BMDM))
Specification Bone Marrow derived macrophages (BMDM)
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Miss. Sarah Rodrigues Moreira

Verified customer

提交于 Mar 01 2013

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. We have not had a band reported to use of this size, the predicted MW is around 68 kD but we saw the band a bit higher at 100 kD. We do not have t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549)
Loading amount 15 µg
Specification A549
Treatment 50 uM BSO
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Amelia Romoser

Verified customer

提交于 Jan 28 2011

Application Western blot
Sample Human Cell lysate - whole cell (Human Mammary Epithelial Cells (HMEC))
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 10000 cells
Specification Human Mammary Epithelial Cells (HMEC)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 22 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain tissue)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 50 µg
Specification Brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 31 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (brain)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 50 µg
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 26 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Nov 03 2015

Application Western blot
Sample Human Cell lysate - whole cell (Placenta)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 30 µg
Specification Placenta
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Vicky

Verified customer

提交于 Sep 30 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human dermal fibroblasts)
Permeabilization No
Specification Human dermal fibroblasts
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Dr. Kate Hawkins

Verified customer

提交于 Jun 18 2015

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"