概述

  • 产品名称
  • 描述
    兔多克隆抗体to Nrf2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Hamster, Dog, Human
    预测可用于: Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide:

    SQRRKEYELEKQKKLEKERQE

    conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 40-60 of Human Nrf2

  • 阳性对照
    • Rat liver microsomes, Mouse liver microsomes, Human liver microsomes, Canine liver microsomes and Hela cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab92946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/1000 (ECL). Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
    • 组织特异性
      Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
    • 序列相似性
      Belongs to the bZIP family. CNC subfamily.
      Contains 1 bZIP domain.
    • 结构域
      Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
    • 翻译后修饰
      Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
    • 细胞定位
      Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
    • Information by UniProt
    • 数据库链接
    • 别名
      • erythroid derived 2 antibody
      • HEBP1 antibody
      • like 2 antibody
      • NF E2 related factor 2 antibody
      • NF-E2-related factor 2 antibody
      • NF2L2_HUMAN antibody
      • NFE2 related factor 2 antibody
      • NFE2-related factor 2 antibody
      • Nfe2l2 antibody
      • Nrf 2 antibody
      • NRF2 antibody
      • Nuclear factor (erythroid derived 2) like 2 antibody
      • Nuclear factor antibody
      • nuclear factor erythroid 2 like 2 antibody
      • Nuclear factor erythroid 2 related factor 2 antibody
      • Nuclear factor erythroid 2-related factor 2 antibody
      • Nuclear factor erythroid derived 2 like 2 antibody
      see all

    图片

    • All lanes : Anti-Nrf2 antibody (ab92946) at 1/1000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Rat liver microsomes
      Lane 3 : Mouse liver microsomes
      Lane 4 : Human liver microsomes
      Lane 5 : Canine liver microsomes
      Lane 6 : HeLa cell lysate

      Developed using the ECL technique

      Predicted band size : 70 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    This product has been referenced in:
    • Soares MA  et al. Restoration of Nrf2 Signaling Normalizes the Regenerative Niche. Diabetes 65:633-46 (2016). Read more (PubMed: 26647385) »
    • Meng X  et al. Hydrogen-rich saline attenuates chemotherapy-induced ovarian injury via regulation of oxidative stress. Exp Ther Med 10:2277-2282 (2015). WB . Read more (PubMed: 26668628) »

    See all 2 Publications for this product

    客户评价及客户问答

    Thank you for contacting us and for your interest in our products.

    As far as we are aware, ab92946 has never been tested in ChIP. All tested applications are specified on our datasheets, and these are updated as soon as any new information i...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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