概述

  • 产品名称Anti-Nrf2抗体
    参阅全部 Nrf2 一抗
  • 描述
    兔多克隆抗体to Nrf2
  • 经测试应用适用于: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow
  • 免疫原

    Synthetic peptide:

    TL YLEVFSMLRD EDGKPYSP

    , corresponding to amino acids 569-588 of Human Nrf2

  • 阳性对照
    • Placenta

性能

应用

Our Abpromise guarantee covers the use of ab31163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 17142801
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). See Abreview.

靶标

  • 功能Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • 组织特异性Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • 序列相似性Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • 结构域Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • 翻译后修饰Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • 细胞定位Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • 数据库链接
  • 别名
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

Anti-Nrf2 antibody 图像

  • ab31163 staining Nrf2 in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab31163 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31163, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Nrf2 antibody (ab31163) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 28 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Immunohistochemical analysis of frozen mouse retinal tissue, labelling Nrf2 with ab31163 at a dilution of 1/100 incubated for 2 hours at 21°C. Permeabilization was via additon of 0.5% Triton X-100. Blocking was with 1% BSA for 1 hour at 21°C. The secondary used was ab150086 used at 1/600.

    See Abreview

  • ab31163 staining Nrf2 in Mouse abdominal wall tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab31163 at 1/100 dilution, staining Nrf2 in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

Anti-Nrf2 antibody (ab31163)参考文献

This product has been referenced in:
  • Guo C  et al. Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats. J Ethnopharmacol 151:660-6 (2014). WB, IHC-Fr ; Rat . Read more (PubMed: 24291152) »
  • Jo C  et al. Nrf2 reduces levels of phosphorylated tau protein by inducing autophagy adaptor protein NDP52. Nat Commun 5:3496 (2014). Read more (PubMed: 24667209) »

See all 19 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (OVCAR3 cells)
Gel Running Conditions Reduced Denaturing (4-20% Tris-Glycin gel)
Loading amount 300000 cells
Specification OVCAR3 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 06 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (BxPC3 - pancreatic cancer cell line)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification BxPC3 - pancreatic cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 18 2016

Application Western blot
Sample Mouse Tissue lysate - other (liver cytosol)
Gel Running Conditions Reduced Denaturing (gel 10%)
Loading amount 40 µg
Treatment hyperoxia (95%O2) for 44 hours
Specification liver cytosol
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: +4°C
Username

Dr. Sandra Sobocanec

Verified customer

提交于 Oct 06 2015

Application Western blot
Sample Mouse Tissue lysate - nuclear (retina)
Gel Running Conditions Non-reduced Denaturing
Loading amount 20 µg
Specification retina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Oct 01 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (retina)
Permeabilization Yes - 0.5% Triton X-100
Specification retina
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative fresh frozen
Username

Abcam user community

Verified customer

提交于 Aug 17 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - nuclear (Spinal cord lysate)
Specification Spinal cord lysate
Blocking step Licor Blocking buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Username

Abcam user community

Verified customer

提交于 Jan 14 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (brain)
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 22 2014



I believe that the Nrf2 that does enter the nucleus is acetylated and phosphorylated, which will make the protein run more slowly through the SDS PAGE gel, making its apparent molecular weight seem larger than based on theoretical amino acid ...

Read More
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (HepG2 cell lysate-ab7900)
Specification HepG2 cell lysate-ab7900
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 07 2014

After review of your protocol, at this time, I would recommend use of 3-5% BSA in TBST blocking solution or to use a casein block. There are two other possibilities which could help as well. The first is that there may be too much protein per well. ...

Read More

1-10 of 40 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"