概述

  • 产品名称
  • 描述
    兔多克隆抗体to Nrf2
  • 经测试应用
    适用于: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow
  • 免疫原

    Synthetic peptide:

    TL YLEVFSMLRD EDGKPYSP

    , corresponding to amino acids 569-588 of Human Nrf2

  • 阳性对照
    • Placenta

性能

应用

Our Abpromise guarantee covers the use of ab31163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 17142801
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).Can be blocked with ab224517. See Abreview.

靶标

  • 功能
    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • 组织特异性
    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • 序列相似性
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • 结构域
    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • 翻译后修饰
    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • 细胞定位
    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • 数据库链接
  • 别名
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

Anti-Nrf2 antibody 图像

  • ab31163 staining Nrf2 in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab31163 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31163, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Nrf2 antibody (ab31163) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 28 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Immunohistochemical analysis of frozen mouse retinal tissue, labelling Nrf2 with ab31163 at a dilution of 1/100 incubated for 2 hours at 21°C. Permeabilization was via additon of 0.5% Triton X-100. Blocking was with 1% BSA for 1 hour at 21°C. The secondary used was ab150086 used at 1/600.

    See Abreview

  • ab31163 staining Nrf2 in Mouse abdominal wall tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab31163 at 1/100 dilution, staining Nrf2 in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

Anti-Nrf2 antibody (ab31163)参考文献

This product has been referenced in:
  • Guo C  et al. Protocatechualdehyde Protects Against Cerebral Ischemia-Reperfusion-Induced Oxidative Injury Via Protein Kinase Ce/Nrf2/HO-1 Pathway. Mol Neurobiol 54:833-845 (2017). Read more (PubMed: 26780453) »
  • Singh A  et al. Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen. Front Immunol 8:183 (2017). Read more (PubMed: 28289414) »

See all 33 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (OVCAR3 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gel)
Loading amount
300000 cells
Specification
OVCAR3 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 06 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (BxPC3 - pancreatic cancer cell line)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
BxPC3 - pancreatic cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 18 2016

Application
Western blot
Sample
Mouse Tissue lysate - other (liver cytosol)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
40 µg
Treatment
hyperoxia (95%O2) for 44 hours
Specification
liver cytosol
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: +4°C
Username

Dr. Sandra Sobocanec

Verified customer

提交于 Oct 06 2015

Application
Western blot
Sample
Mouse Tissue lysate - nuclear (retina)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Oct 01 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (retina)
Permeabilization
Yes - 0.5% Triton X-100
Specification
retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
fresh frozen
Username

Abcam user community

Verified customer

提交于 Aug 17 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - nuclear (Spinal cord lysate)
Specification
Spinal cord lysate
Blocking step
Licor Blocking buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Username

Abcam user community

Verified customer

提交于 Jan 14 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (brain)
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 22 2014



I believe that the Nrf2 that does enter the nucleus is acetylated and phosphorylated, which will make the protein run more slowly through the SDS PAGE gel, making its apparent molecular weight seem larger than based on theoretical amino acid ...

Read More
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (HepG2 cell lysate-ab7900)
Specification
HepG2 cell lysate-ab7900
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 07 2014

After review of your protocol, at this time, I would recommend use of 3-5% BSA in TBST blocking solution or to use a casein block. There are two other possibilities which could help as well. The first is that there may be too much protein per well. ...

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