Anti-NRF1抗体- ChIP Grade (ab34682)

概述

  • 产品名称Anti-NRF1抗体- ChIP Grade
    参阅全部 NRF1 一抗
  • 描述
    兔多克隆抗体to NRF1 - ChIP Grade
  • 特异性ab34682 recognises NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal).
  • 经测试应用适用于: ChIP, WB, IHC-P, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Dog, Chimpanzee, Zebrafish
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-534 of Mouse NRF1

  • 阳性对照
    • HeLa Whole Cell Lysate HeLa Cell Nuclear Extract Human testis

性能

应用

Our Abpromise guarantee covers the use of ab34682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
WB 1/500 - 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
IHC-P Use a concentration of 2 - 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/3000 - 1/10000.
EMSA Use at an assay dependent concentration. PubMed: 21609478

靶标

Anti-NRF1 antibody - ChIP Grade 图像

  • All lanes : Anti-NRF1 antibody - ChIP Grade (ab34682) at 1/1000 dilution

    Lane 1 : Mouse liver whole tissue lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 55,67 kDa (why is the actual band size different from the predicted?)


    Exposure time : 50 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-NRF1 antibody - ChIP Grade (ab34682) at 1/500 dilution

    Lane 1 : HeLa Nuclear extract
    Lane 2 : HeLa Whole Cell lysate

    Secondary
    IRDyeTM700 labelled goat-a-rabbit IgG at 1/2500 dilution

    Predicted band size : 57 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)
  • Purified anti-NRF1 antibody (ab34682) was used at 5µg/ml to detect NRF1 in human testis. Nuclear staining in lymphocytes can be observed.
  • ICC/IF image of ab34682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NRF1 antibody - ChIP Grade (ab34682)参考文献

This product has been referenced in:
  • Hashimoto T  et al. A synergistic DNA logic predicts genome-wide chromatin accessibility. Genome Res 26:1430-1440 (2016). Read more (PubMed: 27456004) »
  • Gao G & Moyes CD Evaluating the role of NRF-1 in the regulation of the goldfish COX4-1 gene in response to temperature. J Exp Biol 219:3019-3027 (2016). Read more (PubMed: 27471277) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 19 2014

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab34682.
To check the status of the order please contact our Cus...

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Vielen Dank für Ihre Nachricht! Es freut mich zu hören, dass das neue Röhrchen ein gutes Signal gibt auf dem Mausgewebe.

Es ist erstaunlich, dass das Signal auf dem Muskel nicht mehr da ist. In der Tat ist das Protein vielleich...

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Vielen Dank für Ihren Anruf.

Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben mit einem neuen Röhrchen einer anderen Abpackung von ab34682.Die Bestellunghat die Referenznummer 10733...

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Vielen Dank für Ihre Antwort.

Es tut mir leid, dass der ab34682 kein Signal mehr gibt. Leider kann ich mir nicht erklären, wieso dies so ist. Wir werden Ihnen gerne einen Ersatzantikörper senden. Könnten Sie bitte hierzu ...

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Thank you for your reply.

Please find the information below regarding the testing discount offer, to test ab90425 in ChIP. Also I have sent you ab90425 as a free of charge replacement for ab34682 which was mislabeled on our website. There sho...

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Thank you for contacting Abcam. Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:

- Return in the original Abcam box with the coo...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital i...

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Application Western blot
Sample Mouse Tissue lysate - whole (liver, muscle)
Loading amount 20 µg
Specification liver, muscle
Gel Running Conditions Reduced Denaturing (4-12% BioRad Criterion XT)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 26 2009

Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12)
Loading amount 15 µg
Specification C2C12
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"