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Recombinant fragment (GST-tag) corresponding to Human NRF1 aa 201-285.
Our Abpromise guarantee covers the use of ab55744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 54 kDa.|
|IHC-P||Use a concentration of 3 µg/ml.|
|IP||Use at an assay dependent concentration. PubMed: 24250222|
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Western blot analysis of HEK293 whole cell lysate (30μg/lane) labelling NRF1 with ab55744 at 1/1000 in5% milk for 16 hours at 4ºC. A IRDye® 680-conjugated goat anti-mouse polyclonal (1/4000) was used as the secondary antibody. Exposure was 5 minutes and Licor scan dection was used.
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