概述

  • 产品名称Anti-NRAS抗体
    参阅全部 NRAS 一抗
  • 描述
    山羊多克隆抗体to NRAS
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Guinea pig, Cow, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide:

    C-RMKKLNSSDDGTQ

    , corresponding to internal sequence amino acids 167-179 of Human NRAS (NP_002515.1).

  • 阳性对照
    • A431 lysate, human small intestine, rat intestine.

性能

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应用

Our Abpromise guarantee covers the use of ab77392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 - 3 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

靶标

  • 功能Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • 疾病相关Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
    Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
  • 序列相似性Belongs to the small GTPase superfamily. Ras family.
  • 翻译后修饰Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
  • 细胞定位Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALPS4 antibody
    • AV095280 antibody
    • GTPase NRas antibody
    • HRAS1 antibody
    • N ras antibody
    • N ras protein part 4 antibody
    • Neuroblastoma RAS viral (v ras) oncogene homolog antibody
    • NRAS antibody
    • NRAS1 antibody
    • NS6 antibody
    • OTTHUMP00000013879 antibody
    • OTTMUSP00000023521 antibody
    • RASN_HUMAN antibody
    • Transforming protein N Ras antibody
    • Transforming protein N-Ras antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

Anti-NRAS antibody 图像

  • Anti-NRAS antibody (ab77392) at 0.01 µg/ml + A431 lysate (in RIPA buffer) at 35 µg

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • ab77392 at 2.5 µg/ml staining NRAS in human small intestine tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • Anti-NRAS antibody (ab77392) at 0.1 µg/ml + Rat intestine lysate in RIPA buffer. at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

Anti-NRAS antibody (ab77392)参考文献

This product has been referenced in:
  • Sun D  et al. RAS/MEK-independent gene expression reveals BMP2-related malignant phenotypes in the Nf1-deficient MPNST. Mol Cancer Res 11:616-27 (2013). Read more (PubMed: 23423222) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jun 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"