概述

  • 产品名称
  • 描述
    山羊多克隆抗体to NRAS
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Guinea pig, Dog, Pig, Opossum, Orangutan
  • 免疫原

    Synthetic peptide:

    C-RMKKLNSSDDGTQ

    , corresponding to internal sequence amino acids 167-179 of Human NRAS (NP_002515.1).

  • 阳性对照
    • Human Small Intestine tissue and A431 lysate.

性能

应用

Our Abpromise guarantee covers the use of ab118814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • 疾病相关
    Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
    Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
  • 序列相似性
    Belongs to the small GTPase superfamily. Ras family.
  • 翻译后修饰
    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
  • 细胞定位
    Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALPS4 antibody
    • AV095280 antibody
    • GTPase NRas antibody
    • HRAS1 antibody
    • N ras antibody
    • N ras protein part 4 antibody
    • Neuroblastoma RAS viral (v ras) oncogene homolog antibody
    • NRAS antibody
    • NRAS1 antibody
    • NS6 antibody
    • OTTHUMP00000013879 antibody
    • OTTMUSP00000023521 antibody
    • RASN_HUMAN antibody
    • Transforming protein N Ras antibody
    • Transforming protein N-Ras antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

图片

  • ab118814, at 2.5 µg/ml, staining NRAS in formalin-fixed, paraffin-embedded Human Small Intestine tissue by Immunohistochemistry using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-NRAS antibody (ab118814) at 0.01 µg/ml + A431 lysate in RIPA buffer at 35 µg

    Predicted band size : 21 kDa

文献

ab118814 has not yet been referenced specifically in any publications.

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