产品名称 Anti-NRAP抗体 参阅全部 NRAP 一抗
IHC-P, ICC/IF more details
internal sequence amino acids 1309-1393 (RHDFVKERGK LIGPQSVRDD PRIQHCRRMG QLQSELQYRR GATSSQAQFH LPMDMVHLVH AKNAQALASD HDYRTQYHKF TALPE) of the Human NRAP protein (UniProt ID: Q86VF7).
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 pH: 5.20 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
功能 May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.
组织特异性 Expressed in cardiac and skeletal muscle.
序列相似性 Contains 1 LIM zinc-binding domain. Contains 44 nebulin repeats.
细胞定位 Localized at the myotendinous junction in skeletal muscle and at the intercalated disk in cardiac muscle.
Information by UniProt
Nebulin-related-anchoring protein antibody
Anti-NRAP antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-NRAP antibody (ab122427)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, centrosome and cell junctions. Recommended concentration of ab122427 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (ab122427)
ab122427, at a 1/200 dilution, staining NRAP in paraffin embedded Human liver tissue by Immunohistochemistry.
Western blot - Anti-NRAP antibody (ab122427) This image is courtesy of an anonymous abreview.
Anti-NRAP antibody (ab122427) at 1/1000 dilution + Sheep cardiac tissue at 10 µg Secondary Donkey Anti-Rabbit IgG (H+L) at 1/20000 dilution Developed using the ECL technique Performed under reducing conditions. Observed band size : 190 kDa ( why is the actual band size different from the predicted?) Exposure time : 50 seconds This image is courtesy of an anonymous abreview.
Anti-NRAP antibody (ab122427)参考文献
has not yet been referenced specifically in any publications.
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