Anti-NRAP抗体(ab122427)

概述

  • 产品名称Anti-NRAP抗体
    参阅全部 NRAP 一抗
  • 描述
    兔多克隆抗体to NRAP
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Sheep, Human
  • 免疫原

    internal sequence amino acids 1309-1393 (RHDFVKERGK LIGPQSVRDD PRIQHCRRMG QLQSELQYRR GATSSQAQFH LPMDMVHLVH AKNAQALASD HDYRTQYHKF TALPE) of the Human NRAP protein (UniProt ID: Q86VF7).

  • 阳性对照
    • Human liver tissue

性能

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应用

Our Abpromise guarantee covers the use of ab122427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 功能May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.
  • 组织特异性Expressed in cardiac and skeletal muscle.
  • 序列相似性Contains 1 LIM zinc-binding domain.
    Contains 44 nebulin repeats.
  • 细胞定位Localized at the myotendinous junction in skeletal muscle and at the intercalated disk in cardiac muscle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • N-RAP antibody
    • Nebulin-related-anchoring protein antibody
    • Nrap antibody
    • NRAP_HUMAN antibody

Anti-NRAP antibody 图像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, centrosome and cell junctions. Recommended concentration of ab122427 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122427, at a 1/200 dilution, staining NRAP in paraffin embedded Human liver tissue by Immunohistochemistry.
  • Anti-NRAP antibody (ab122427) at 1/1000 dilution + Sheep cardiac tissue at 10 µg

    Secondary
    Donkey Anti-Rabbit IgG (H+L) at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 190 kDa (why is the actual band size different from the predicted?)


    Exposure time : 50 seconds

    This image is courtesy of an anonymous abreview.

    See Abreview

Anti-NRAP antibody (ab122427)参考文献

ab122427 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Sheep Tissue lysate - other (cardiac)
Specification cardiac
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Dec 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"