概述

  • 产品名称Anti-NRAMP1抗体
    参阅全部 NRAMP1 一抗
  • 描述
    小鼠单克隆抗体to NRAMP1
  • 经测试应用适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: QAFYQKTNQA AFNICANSSL HDYAKIFPMN NATVAVDIYQ GGV, corresponding to amino acids 308-351 of Human NRAMP1

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: A549

性能

应用

Our Abpromise guarantee covers the use of ab58138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.
  • 组织特异性Macrophages; peripheral blood leukocytes, lung, spleen and liver.
  • 序列相似性Belongs to the NRAMP family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LSH antibody
    • Natural resistance associated macrophage protein 1 antibody
    • Natural resistance-associated macrophage protein 1 antibody
    • NRAM1_HUMAN antibody
    • NRAMP 1 antibody
    • NRAMP antibody
    • PBC antibody
    • SLC11A1 antibody
    • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 1 antibody
    • solute carrier family 11 (sodium/phosphate symporters) member 1 antibody
    • Solute carrier family 11 member 1 antibody
    see all

Anti-NRAMP1 antibody 图像

  • Overlay histogram showing HepG2 cells stained with ab58138 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58138, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • ICC/IF image of ab58138 stained A549 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58138 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot against tagged recombinant protein immunogen using ab58138 NRAMP1 antibody at 1ug/ml. Predicted band size of immunogen is 31 kDa

Anti-NRAMP1 antibody (ab58138)参考文献

ab58138 has not yet been referenced specifically in any publications.

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