The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.
Use a concentration of 0.002 µg/ml.
Use at an assay dependent dilution.
功能Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
组织特异性Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
序列相似性Belongs to the nuclear hormone receptor family. NR5 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Nuclear receptor subfamily 5 group A member 2 antibody
Anti-NR5A2 / LRH1 antibody [H2325] 图像
Western blot - Anti-NR5A2 / LRH1 antibody [H2325] (ab41901)This image is courtesy of an Abreview submitted by Chun Fai Lai.
All lanes : Anti-NR5A2 / LRH1 antibody [H2325] (ab41901) at 1/1000 dilution
Lane 1 : COS-1 cells transfected with NR5A2 Lane 2 : COS-1 cells transfected with NR5A2 Lane 3 : COS-1 cells transfected with NR5A2 Lane 4 : COS-1 cells transfected with NR5A2 Lane 5 : COS-1 cells transfected with NR5A2 Lane 6 : COS-1 cells transfected with NR5A2 Lane 7 : COS-1 cells transfected with NR5A2 Lane 8 : COS-1 cells transfected with NR5A2
Lysates/proteins at 15 µg per lane.
Secondary HRP-conjugated Goat anti-Mouse IgG polyclonal at 1/2500 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 61 kDa Observed band size : 61 kDa
Exposure time : 10 seconds
This image is courtesy of an Abreview submitted by Chun Fai Lai.
Duggan SP et al. The characterization of an intestine-like genomic signature maintained during Barrett's-associated adenocarcinogenesis reveals an NR5A2-mediated promotion of cancer cell survival. Sci Rep6:32638 (2016).
Read more (PubMed: 27586588) »
Kramer HB et al. LRH-1 drives colon cancer cell growth by repressing the expression of the CDKN1A gene in a p53-dependent manner. Nucleic Acids Res44:582-94 (2016).
Read more (PubMed: 26400164) »