概述

  • 产品名称Anti-NR2F6抗体
    参阅全部 NR2F6 一抗
  • 描述
    兔多克隆抗体to NR2F6
  • 经测试应用适用于: ELISA, ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from N-terminal of human NR2F6.

  • 阳性对照
    • HeLa, 293, HUVEC or HepG2 cells. Cervix carcinoma tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab65012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).
  • 组织特异性Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EAR 2 antibody
    • EAR-2 antibody
    • EAR2 antibody
    • ERBA RELATED 2 antibody
    • ERBA related gene 2 antibody
    • ERBAL2 antibody
    • Nr2f6 antibody
    • NR2F6_HUMAN antibody
    • Nuclear receptor subfamily 2 group F member 6 antibody
    • Orphan nuclear receptor EAR2 (V erbA related protein EAR 2) antibody
    • v erb a avian erythroblastic leukemia viral oncogene homolog like antibody
    • V erbA related protein EAR 2 antibody
    • V-erbA-related protein 2 antibody
    see all

Anti-NR2F6 antibody 图像

  • ab65012 at 1/500 - 1/1000 dilution staining NR2F6 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab65012 at 1/50 - 1/100 dilution staining human cervix carcinoma in NR2F6 by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-NR2F6 antibody (ab65012) at 1/500 dilution

    Lane 1 : HeLa cell extract at 10-30µg
    Lane 2 : 293 cell extract at 10-30µg
    Lane 3 : HUVEC cell extract at 10-30µg
    Lane 4 : HUVEC cell extract at 10-30µg with immunizing peptide at 5-10µg


    Predicted band size : 43 kDa
    Observed band size : 43 kDa

Anti-NR2F6 antibody (ab65012)参考文献

This product has been referenced in:
  • Weatherford ET  et al. Regulation of renin expression by the orphan nuclear receptors Nr2f2 and Nr2f6. Am J Physiol Renal Physiol 302:F1025-33 (2012). WB . Read more (PubMed: 22278040) »
  • Modica S  et al. The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors. Gastroenterology 138:636-48, 648.e1-12 (2010). IHC-P ; Human . Read more (PubMed: 19818784) »

See all 2 Publications for this product

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