Anti-NR2F2抗体[H7147] (ab41859)

概述

  • 产品名称Anti-NR2F2抗体[H7147]
    参阅全部 NR2F2 一抗
  • 描述
    小鼠单克隆抗体[H7147] to NR2F2
  • 特异性This antibody does not recognize human COUP TF1 and EAR2.
  • 经测试应用适用于: ELISA, IHC-P, IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow
  • 免疫原

    Recombinant fragment:

    PGQGGPAS TPAQTAAGGQ GGPG

    , corresponding to amino acids 43-64 of Human NR2F2

  • 阳性对照
    • Human Convoluted tubule and Rat Glomerular

应用

Our Abpromise guarantee covers the use of ab41859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
ICC/IF 1/1000.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).

靶标

Anti-NR2F2 antibody [H7147] 图像

  • Immnocytochemical immunoflurescence analysis of PFA-fixed human pluripotent stem cell derived cardiomyocytes, labelling NR2F2 with ab41859 at a concentration of 1/1000 incubated for 16 hours at 4°C. Permeablisation is using saponin. Blocking was done with 5% serum for 15 minutes at room temperature. Secondary used was a goat anti-mouse Alexa Fluor® 568 at 1/2000. The left panel shows the experimental conditions described formerly. The right panel is additionally counterstained with DAPI to show all cell nuclei within the area.

    See Abreview

  • ab41859 staining human NR2F2 in convoluted tubule by Immunohistochemistry (FFPE-sections) 10ug/ml.
  • ab41859 staining rat NR2F2 in Glomerular by Immunohistochemistry (FFPE-sections) 10ug/ml.
  • All lanes : Anti-NR2F2 antibody [H7147] (ab41859) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-NR2F2 antibody [H7147] (ab41859)参考文献

This product has been referenced in:
  • Kim BJ & Scott DA Mouse model reveals the role of RERE in cerebellar foliation and the migration and maturation of Purkinje cells. PLoS One 9:e87518 (2014). IHC-P ; Mouse . Read more (PubMed: 24466353) »
  • Chan CM  et al. A signature motif mediating selective interactions of BCL11A with the NR2E/F subfamily of orphan nuclear receptors. Nucleic Acids Res N/A:N/A (2013). Read more (PubMed: 23975195) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human pluripotent stem cell derived cardiomyocytes)
Permeabilization Yes - saponin
Specification human pluripotent stem cell derived cardiomyocytes
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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提交于 Aug 20 2015

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I am very pleased to hear you would like to accept our offer and test ab41859 in ChIP. This code will give you €370 off your nex...

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Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab41859 NR2F2 antibody [H7147] has never been tested in ChIP. All tested applications are specified on our datasheets, and these are updated as soon as any new in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"