Anti-NR2F2抗体[H7147] (ab41859)
Key features and details
- Mouse monoclonal [H7147] to NR2F2
- Suitable for: IP, ELISA, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
概述
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产品名称
Anti-NR2F2抗体[H7147]
参阅全部 NR2F2 一抗 -
描述
小鼠单克隆抗体[H7147] to NR2F2 -
宿主
Mouse -
经测试应用
适用于: IP, ELISA, IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow -
免疫原
Recombinant fragment:
PGQGGPAS TPAQTAAGGQ GGPG
, corresponding to amino acids 43-64 of Human NR2F2 -
阳性对照
- Human Convoluted tubule and Rat Glomerular
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常规说明
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Physiological saline. -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
H7147 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41859于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
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ICC/IF | (1) |
Use at an assay dependent concentration.
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说明 |
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IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa). |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. -
组织特异性
Ubiquitous. -
疾病相关
Congenital heart defects, multiple types, 4 -
序列相似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 281945 Cow
- Entrez Gene: 7026 Human
- Entrez Gene: 11819 Mouse
- Entrez Gene: 113984 Rat
- Omim: 107773 Human
- SwissProt: Q9TTR7 Cow
- SwissProt: P24468 Human
- SwissProt: P43135 Mouse
see all -
别名
- Apolipoprotein A-I regulatory protein 1 antibody
- Apolipoprotein AI regulatory protein 1 antibody
- ARP-1 antibody
see all
图片
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ab41859 staining human NR2F2 in convoluted tubule by Immunohistochemistry (FFPE-sections) 10ug/ml.
This image was generated using the ascites version of the product.
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ab41859 staining rat NR2F2 in Glomerular by Immunohistochemistry (FFPE-sections) 10ug/ml.
This image was generated using the ascites version of the product.
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All lanes : Anti-NR2F2 antibody [H7147] (ab41859) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab41859 被引用在 23 文献中.
- Polvani S et al. Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression. Int J Oncol 60:N/A (2022). PubMed: 35348189
- Meçe O et al. Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis. Nat Commun 13:2760 (2022). PubMed: 35589749
- Wei JR et al. Identification of visual cortex cell types and species differences using single-cell RNA sequencing. Nat Commun 13:6902 (2022). PubMed: 36371428
- O'Leary TP et al. Neuronal cell types, projections, and spatial organization of the central amygdala. iScience 25:105497 (2022). PubMed: 36425768
- Aguiari P et al. Persistent COUP-TFII expression underlies the myopathy and impaired muscle regeneration observed in resistance to thyroid hormone-alpha. Sci Rep 11:4601 (2021). PubMed: 33633251