概述

  • 产品名称
  • 描述
    兔多克隆抗体to NR2F2
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • 免疫原

    A region within synthetic peptide: KVGMRREAVQ RGRMPPTQPT HGQFALTNGD PLNCHSYLSG YISLLLRAEP, corresponding to amino acids 141-190 of Human NR2F2

  • 阳性对照
    • Transfected 293T cell lysate; Epithelial cells of renal tubule of human kidney

应用

Our Abpromise guarantee covers the use of ab50487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

图片



  • Predicted band size : 46 kDa
    Western blot analysis of MCF7 whole cell lysate labelling NR2F2 with ab50487 at 1.0µg/ml.
  • Lane 1 : Marker
    Lane 2 : Anti-NR2F2 antibody (ab50487) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : Transfected 293T cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffine-embedded sections) analysis of human kidney tissue labelling NRF2 with ab50487 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400x.

文献

This product has been referenced in:
  • Chen X  et al. Expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs. J Anim Sci Biotechnol 7:31 (2016). WB, IHC . Read more (PubMed: 27231548) »
  • Wang C  et al. High expression of COUP-TF II cooperated with negative Smad4 expression predicts poor prognosis in patients with colorectal cancer. Int J Clin Exp Pathol 8:7112-21 (2015). IHC . Read more (PubMed: 26261604) »

See all 3 Publications for this product

客户评价及客户问答

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human pluripotent stem cell derived cardiomyocytes)
Specification
human pluripotent stem cell derived cardiomyocytes
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册