概述

  • 产品名称
    Anti-NR2E1 / Tailless抗体
    参阅全部 NR2E1 / Tailless 一抗
  • 描述
    兔多克隆抗体to NR2E1 / Tailless
  • 经测试应用
    适用于: ChIP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide derived from residues 95 - 144 of human NR2E1.

  • 阳性对照
    • Jurkat cell lysate; human skin tissue.

性能

应用

Our Abpromise guarantee covers the use of ab30942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use a concentration of 4 µg/ml. PubMed: 19505455
WB Use a concentration of 0.03 - 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

Anti-NR2E1 / Tailless antibody 图像

  • Immunohistochemical analysis of NR2E1 expression in paraffin embedded human skin tissue section, using 4 µg/ml ab30942. Arrows indicate positively stained squamous epithelial cells.
  • Lane 1 : Anti-NR2E1 / Tailless antibody (ab30942) at 0.25 µg/ml
    Lane 2 : Anti-NR2E1 / Tailless antibody (ab30942) at 0.125 µg/ml

    Lane 1 : Cell lysate prepared from human Jurkat cells
    Lane 2 : Cell lysate prepared from human Jurkat cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 42 kDa

Anti-NR2E1 / Tailless antibody (ab30942)参考文献

This product has been referenced in:
  • Fowler PA  et al. In utero exposure to cigarette smoke dysregulates human fetal ovarian developmental signalling. Hum Reprod N/A:N/A (2014). Human . Read more (PubMed: 24847019) »
  • Sehgal R  et al. BMP7 and SHH regulate Pax2 in mouse retinal astrocytes by relieving TLX repression. Dev Biol 332:429-43 (2009). ChIP . Read more (PubMed: 19505455) »

See all 2 Publications for this product

Product Wall

Application
Western blot
Sample
Rabbit Tissue lysate - whole (rabbit brain tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
rabbit brain tissue
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Praveen Ballabh

Verified customer

提交于 Nov 10 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Developing brain E12-P0)
Specification
Developing brain E12-P0
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
Yes - 0.1%Triton, 0.1% Tween
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Dr. Paula Nichols

Verified customer

提交于 Dec 14 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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