RabMAb

Anti-NR2C2抗体[EPR1773(2)] (ab109301)

概述

  • 产品名称Anti-NR2C2抗体[EPR1773(2)]
    参阅全部 NR2C2 一抗
  • 描述
    兔单克隆抗体[EPR1773(2)] to NR2C2
  • 经测试应用适用于: WB, IPmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NR2C2 aa 500 to the C-terminus.

  • 阳性对照
    • PC3 cell lysate, 293T cell lysate; Human testis tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa).
IP 1/10 - 1/100.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
    • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 发展阶段Transiently repressed during the meiotic phase of spermatogenesis.
    • 翻译后修饰Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 形式There are 2 isoforms produced by alternative splicing.
    • 别名
      • hTAK1 antibody
      • Nr2c2 antibody
      • NR2C2_HUMAN antibody
      • Nuclear hormone receptor TR4 antibody
      • Nuclear receptor subfamily 2 group C member 2 antibody
      • Orphan nuclear receptor TAK1 antibody
      • Orphan nuclear receptor TR4 antibody
      • TAK1 antibody
      • Testicular nuclear receptor 4 antibody
      • Testicular receptor 4 antibody
      • TR2R1 antibody
      • TR4 antibody
      • TR4 nuclear hormone receptor antibody
      see all

    Anti-NR2C2 antibody [EPR1773(2)] 图像

    • All lanes : Anti-NR2C2 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified)

      Lane 1 : PC-12 whole cell lysate
      Lane 2 : 3T3-L1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 65 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-NR2C2 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified) + HEK293 whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 65 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-NR2C2 antibody [EPR1773(2)] (ab109301) at 1/2000 dilution (purified) + PC-3 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 65 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    • ab109301 (purified) at 1/30 immunoprecipitating NR2C2 in 10 μg HEK293 cell lysate (Lanes 1 and 2, observed at 67 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • All lanes : Anti-NR2C2 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution (Unpurified)

      Lane 1 : PC3 cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)

    Anti-NR2C2 antibody [EPR1773(2)] (ab109301)参考文献

    ab109301 has not yet been referenced specifically in any publications.

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