概述

  • 产品名称Anti-NR2C2抗体
    参阅全部 NR2C2 一抗
  • 描述
    兔多克隆抗体to NR2C2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: HLQNSIQEDK LSGDRIKQVM EHIWKLQEFC NSMAKLDIDG YEYAYLKAIV, corresponding to a region within C terminal amino acids 469-518 of Human NR2C2

  • 阳性对照
    • HepG2 cell lysate. Human skin.

性能

应用

Our Abpromise guarantee covers the use of ab65676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 发展阶段Transiently repressed during the meiotic phase of spermatogenesis.
  • 翻译后修饰Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 形式There are 2 isoforms produced by alternative splicing.
  • 别名
    • hTAK1 antibody
    • Nr2c2 antibody
    • NR2C2_HUMAN antibody
    • Nuclear hormone receptor TR4 antibody
    • Nuclear receptor subfamily 2 group C member 2 antibody
    • Orphan nuclear receptor TAK1 antibody
    • Orphan nuclear receptor TR4 antibody
    • TAK1 antibody
    • Testicular nuclear receptor 4 antibody
    • Testicular receptor 4 antibody
    • TR2R1 antibody
    • TR4 antibody
    • TR4 nuclear hormone receptor antibody
    see all

Anti-NR2C2 antibody 图像

  • Anti-NR2C2 antibody (ab65676) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
  • Immunohistochemical analysis of paraffin-embedded human skin tissue stained with ab65676 at 4µg/ml. Positively stained squamous epithelial cells are indicated with arrows.

Anti-NR2C2 antibody (ab65676)参考文献

ab65676 has not yet been referenced specifically in any publications.

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