Anti-NR0B2抗体[N7519] (ab41947)

概述

  • 产品名称Anti-NR0B2抗体[N7519]
    参阅全部 NR0B2 一抗
  • 描述
    小鼠单克隆抗体[N7519] to NR0B2
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    E.coli expressed recombinant full length protein (Human) NR0B2

性能

应用

Our Abpromise guarantee covers the use of ab41947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at a concentration of 0.5 µg/ml.
    WB: Use at a concentration of 3 µg/ml, under non reducing or reducing conditions. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.
    • 组织特异性Liver. Low levels of expression were detected in heart and pancreas.
    • 疾病相关Obesity
    • 序列相似性Belongs to the nuclear hormone receptor family. NR0 subfamily.
    • 翻译后修饰Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.
    • 细胞定位Nucleus. Cytoplasm. Colocalizes with NEUROD1 in the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • NR0B2 antibody
      • NR0B2_HUMAN antibody
      • Nr0b2a antibody
      • Nuclear receptor subfamily 0 group B member 2 antibody
      • Nuclear receptor subfamily 0, group B, member 2a antibody
      • Orphan nuclear receptor SHP antibody
      • SHP antibody
      • SHP-1 antibody
      • Shp1 antibody
      • Small heterodimer partner antibody
      see all

    Anti-NR0B2 antibody [N7519] (ab41947)参考文献

    ab41947 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (Human hepatocytes in culture)
    Loading amount 10 µg
    Specification Human hepatocytes in culture
    Treatment 50uM chenodeoxycholic acid
    Gel Running Conditions Reduced Denaturing (15% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 12 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"