Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Belongs to the NAD(P)H dehydrogenase (quinone) family.
ICC/IF image of ab93211 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93211, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - NQO2 antibody (ab93211)
Anti-NQO2 antibody (ab93211) at 1/100 dilution + Mouse liver tissue lysate at 35 µg