概述

  • 产品名称
  • 描述
    羊多克隆抗体to NPY1R
  • 特异性
    ab35336 recognises Neuropeptide-Y Receptor 1.
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide: K-Q-A-S-P-V-A-F-K-K-I-N-N-N conjugated to KLH, corresponding to amino acids 365-378 of Human NPY1R

性能

应用

Our Abpromise guarantee covers the use of ab35336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/110.
WB Use at an assay dependent dilution. Predicted molecular weight: 44 kDa.
IHC-P Use at an assay dependent dilution.

靶标

Anti-NPY1R antibody (ab35336)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (rat skin (free floating 50 µm))
Specification
rat skin (free floating 50 µm)
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100, 0.2%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 25 2012

Thank you for your phone call.
I am sorry to hear that the antibody is not working in frozen sections, but unfortunately we have not tried this application and thus do not know for sure if the antibody would work or not in IHC-Fr.

In ord...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain cortex)
Loading amount
100 µg
Specification
brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 8%
Username

Dr. paolo mele

Verified customer

提交于 Feb 13 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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