概述

  • 产品名称Anti-Nptx1抗体
    参阅全部 Nptx1 一抗
  • 描述
    兔多克隆抗体to Nptx1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide within Rat Nptx1 aa 174-203 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.

  • 阳性对照
    • Jurkat cell line lysates, human breast carcinoma tissue and human brain tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab75917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/50 - 1/100. Detects bands of approximately 47 and 60 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Nptx1 antibody 图像

    • Anti-Nptx1 antibody (ab75917) at 1/60 dilution + Jurkat cell line lysates at 35 µg

      Predicted band size : 47 kDa
      Observed band size : 47 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ab75917 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
    • Formalin-fixed and paraffin-embedded human brain tissue reacted with ab75917 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

    Anti-Nptx1 antibody (ab75917)参考文献

    ab75917 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"