Anti-NPTN抗体(ab83063)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to NPTN
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (SAPKANATIE VKAAPDITGH KRSENKNEGQ DATMYCKSVG YPHPDWIWRK) of Human NPTN (NP_059429).

  • 阳性对照
    • Fetal muscle lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity.
  • 组织特异性
    Isoform 1 is ubiquitously expressed. Isoform 2 is expressed in brain cortex and cerebellum (at protein level).
  • 序列相似性
    Contains 3 Ig-like (immunoglobulin-like) domains.
  • 结构域
    Some isoforms lack the first Ig-like domain which may confer homophilic adhesion activity. However, they can bind and activate FGFR1.
  • 翻译后修饰
    N-glycosylated.
  • 细胞定位
    Cell membrane. Enriched at post-synaptic density.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GP55 antibody
    • GP65 antibody
    • Neuroplastin antibody
    • np55 antibody
    • np65 antibody
    • Nptn antibody
    • NPTN_HUMAN antibody
    • SDFR1 antibody
    • SDR-1 antibody
    • SDR1 antibody
    • stromal cell derived factor receptor 1 antibody
    • Stromal cell-derived receptor 1 antibody
    see all

图片

  • Anti-NPTN antibody (ab83063) at 1 µg/ml (5% skim milk / PBS buffer) + Fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab83063 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us about the issues you are having with the FGFR1 and NPTN antibodies. Can you please send me a few details of your samples and the protocols you use for harvesting the samples and for blotting? In particular, I am interested i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain Tissue)
Loading amount
40 µg
Specification
Brain Tissue
Gel Running Conditions
Non-reduced Denaturing (10% Bis-Tris)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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