产品名称 Anti-NPM2抗体 参阅全部 NPM2 一抗
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Full length protein, corresponding to amino acids 1-214 of Human NPM2
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: None
Constituents: PBS, pH 7.2
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纯度 Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 24 kDa).
功能 Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization.
序列相似性 Belongs to the nucleoplasmin family.
结构域 The acidic tract A2 mediates histone binding.
细胞定位 Nucleus. Found in the oocyte nucleus before nuclear membrane breakdown, after which it is redistributed to the cytoplasm.
Information by UniProt
nucleophosmin/nucleoplasmin, 2 antibody
Anti-NPM2 antibody 图像
Western blot - NPM2 antibody (ab67403)
All lanes : Anti-NPM2 antibody (ab67403) at 1/500 dilution Lane 1 : NPM2 transfected 293T cell lysate Lane 2 : NPM2 transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution Predicted band size : 24 kDa Observed band size : 34 kDa ( why is the actual band size different from the predicted?)
Anti-NPM2 antibody (ab67403)参考文献
This product has been referenced in:
et al. Investigating the potential of genes preferentially expressed in oocyte to induce chromatin remodeling in somatic cells. Cell Reprogram 12:519-28 (2010).
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