概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to NPM2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-214 of Human NPM2

  • 阳性对照
    • Transfected 293T cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 24 kDa).

靶标

  • 功能
    Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization.
  • 序列相似性
    Belongs to the nucleoplasmin family.
  • 结构域
    The acidic tract A2 mediates histone binding.
  • 细胞定位
    Nucleus. Found in the oocyte nucleus before nuclear membrane breakdown, after which it is redistributed to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NPM2 antibody
    • NPM2_HUMAN antibody
    • nucleophosmin/nucleoplasmin, 2 antibody
    • nucleoplasmin 2 antibody
    • Nucleoplasmin-2 antibody
    see all

Anti-NPM2 antibody 图像

  • All lanes : Anti-NPM2 antibody (ab67403) at 1/500 dilution

    Lane 1 : NPM2 transfected 293T cell lysate
    Lane 2 : NPM2 transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 24 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-NPM2 antibody (ab67403)参考文献

This product has been referenced in:
  • Sylvestre EL  et al. Investigating the potential of genes preferentially expressed in oocyte to induce chromatin remodeling in somatic cells. Cell Reprogram 12:519-28 (2010). WB ; Human . Read more (PubMed: 20936903) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Mouse Cell (1 cell embryo (Zygote))
Specification
1 cell embryo (Zygote)
Permeabilization
Yes - 0.5% Triton
Fixative
Formaldehyde
Username

Dr. Maki Asami

Verified customer

提交于 Aug 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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