概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to NPL4
  • 特异性
    This antibody is reactive against mammalian transfected lysate.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    NPLOC4 (AAH25930.1, 1 a.a. ~ 608 a.a) full-length human protein.

  • 阳性对照
    • Transfected 293T cell lysate and human spleen tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab68020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/500 - 1/1000. Detects bands of approximately 68 and 75 kDa (predicted molecular weight: 68 kDa).


    ir.Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.
    • 组织特异性
      Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
    • 通路
      Protein degradation; proteasomal ubiquitin-dependent pathway.
    • 序列相似性
      Belongs to the NPL4 family.
      Contains 1 RanBP2-type zinc finger.
    • 结构域
      Binds ubiquitinated proteins via its RanBP2-type zinc finger.
    • 细胞定位
      Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear.
    • Information by UniProt
    • 数据库链接
    • 别名
      • KIAA1499 antibody
      • NPL4 antibody
      • NPL4, S. cerevisiae, homolog of antibody
      • NPL4_HUMAN antibody
      • nploc4 antibody
      • nuclear protein localization 4 homolog (S. cerevisiae) antibody
      • Nuclear protein localization 4 homolog antibody
      • Nuclear protein localization protein 4 homolog antibody
      • Nuclear protein localization protein 4, S. cerevisiae, homolog of antibody
      • Protein NPL4 antibody
      see all

    图片

    • Anti-NPL4 antibody (ab68020) at 1/500 dilution + human spleen tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 68 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-NPL4 antibody (ab68020) at 1/500 dilution

      Lane 1 : NPLOC4 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 68 kDa
      Observed band size : 68 kDa

    实验方案

    文献

    ab68020 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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