Anti-NPL4抗体(ab101226)

概述

  • 产品名称Anti-NPL4抗体
    参阅全部 NPL4 一抗
  • 描述
    兔多克隆抗体to NPL4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat NPL4.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; Caco2; SHSY-5Y; PC3.

性能

应用

Our Abpromise guarantee covers the use of ab101226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

靶标

  • 功能The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.
  • 组织特异性Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
  • 通路Protein degradation; proteasomal ubiquitin-dependent pathway.
  • 序列相似性Belongs to the NPL4 family.
    Contains 1 RanBP2-type zinc finger.
  • 结构域Binds ubiquitinated proteins via its RanBP2-type zinc finger.
  • 细胞定位Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA1499 antibody
    • NPL4 antibody
    • NPL4, S. cerevisiae, homolog of antibody
    • NPL4_HUMAN antibody
    • nploc4 antibody
    • nuclear protein localization 4 homolog (S. cerevisiae) antibody
    • Nuclear protein localization 4 homolog antibody
    • Nuclear protein localization protein 4 homolog antibody
    • Nuclear protein localization protein 4, S. cerevisiae, homolog of antibody
    • Protein NPL4 antibody
    see all

Anti-NPL4 antibody 图像

  • All lanes : Anti-NPL4 antibody (ab101226) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 4 : PC3 whole cell lysate (ab3954)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa


    Exposure time : 8 minutes
  • ICC/IF image of ab101226 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101226 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) Hek293, HepG2, amd MCF-7 cells at 5ug/ml.

Anti-NPL4 antibody (ab101226)参考文献

ab101226 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"