概述

  • 产品名称Anti-NPHS2抗体
    参阅全部 NPHS2 一抗
  • 描述
    兔多克隆抗体to NPHS2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human NPHS2 (C terminal).
    Database link: NM_014625

  • 阳性对照
    • Fetal kidney lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

性能

应用

Our Abpromise guarantee covers the use of ab93650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/200 - 1/500. Predicted molecular weight: 42 kDa.

靶标

  • 功能Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
  • 组织特异性Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
  • 疾病相关Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
  • 序列相似性Belongs to the band 7/mec-2 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
    • nephrosis 2, idiopathic, steroid resistant antibody
    • NPHS2 antibody
    • NPHS2 gene antibody
    • PDCN antibody
    • PODO_HUMAN antibody
    • Podocin antibody
    • SRN1 antibody
    see all

Anti-NPHS2 antibody 图像

  • IHC image of NPHS2 staining in THuman normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93650, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-NPHS2 antibody (ab93650) at 1/200 dilution + Fetal kidney lysate

    Predicted band size : 42 kDa

Anti-NPHS2 antibody (ab93650)参考文献

This product has been referenced in:
  • Wei MG  et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med 2016:5031890 (2016). IHC-P ; Rat . Read more (PubMed: 27403197) »
  • Jeruschke S  et al. Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression. PLoS One 10:e0137043 (2015). IHC-P . Read more (PubMed: 26331477) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab93650.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"