概述

  • 产品名称Anti-NPHS2抗体
    参阅全部 NPHS2 一抗
  • 描述
    兔多克隆抗体to NPHS2
  • 经测试应用适用于: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human NPHS2 aa 367-383 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Synthetic peptide conjugated to KLH by an N-terminal Cysteine residue linker.
    Sequence:

    SKPVEPLNPKKKDSPML

  • 阳性对照
    • Whole extract of rat glomeruli and human or rat kidney frozen sections.

性能

应用

Our Abpromise guarantee covers the use of ab50339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa. Additional weak bands may be detected when immunoblotting various extract preparations. Detection of podocin (doublet) by immunoblotting is specifically inhibited with the immunizing peptide. The upper band may represent a post-translational modified variant.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
  • 组织特异性Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
  • 疾病相关Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
  • 序列相似性Belongs to the band 7/mec-2 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
    • nephrosis 2, idiopathic, steroid resistant antibody
    • NPHS2 antibody
    • NPHS2 gene antibody
    • PDCN antibody
    • PODO_HUMAN antibody
    • Podocin antibody
    • SRN1 antibody
    see all

Anti-NPHS2 antibody 图像

  • ICC/IF image of ab50339 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50339, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NPHS2 antibody (ab50339)参考文献

This product has been referenced in:
  • Al-Trad B  et al. Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats. Diabetol Metab Syndr 7:97 (2015). IHC-P ; Rat . Read more (PubMed: 26583047) »
  • Pippin JW  et al. Cells of renin lineage are adult pluripotent progenitors in experimental glomerular disease. Am J Physiol Renal Physiol 309:F341-58 (2015). ICC/IF ; Mouse . Read more (PubMed: 26062877) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Kidney)
Permeabilization No
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Kidney)
Specification Kidney
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Ms. Jeremy Song

Verified customer

提交于 May 16 2012

Thank you for your reply.
I have issued you the testing discount codes for ab82108 and ab82107 for testing in rat and pig. Please see below for the information.
For ab50339 I am sending you a testing discount code for testing in IHC-P. Since ...

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Thank you for your email.
I am sorry to hear that these antibodies are not working as expected.
As for ab58968, we had replaced this antibody free of charge with ab85379. Since ab85379 was not tested in rat, sheep, and pig tissue, I had sent ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"