概述

  • 产品名称
  • 描述
    兔多克隆抗体to NPHS2
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human NPHS2 aa 367-383 conjugated to keyhole limpet haemocyanin (Cysteine residue). Synthetic peptide conjugated to KLH by an N-terminal Cysteine residue linker.
    Sequence:

    SKPVEPLNPKKKDSPML

  • 阳性对照
    • Whole extract of rat glomeruli and human or rat kidney frozen sections.

性能

应用

Our Abpromise guarantee covers the use of ab50339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa. Additional weak bands may be detected when immunoblotting various extract preparations. Detection of podocin (doublet) by immunoblotting is specifically inhibited with the immunizing peptide. The upper band may represent a post-translational modified variant.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
IHC-P Use a concentration of 0.2 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能
    Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
  • 组织特异性
    Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
  • 疾病相关
    Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
  • 序列相似性
    Belongs to the band 7/mec-2 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
    • nephrosis 2, idiopathic, steroid resistant antibody
    • NPHS2 antibody
    • NPHS2 gene antibody
    • PDCN antibody
    • PODO_HUMAN antibody
    • Podocin antibody
    • SRN1 antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NPHS2 with ab50339 at 0.2 µg/ml.  

     

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NPHS2 with ab50339 at 0.2 µg/ml.  

  • ICC/IF image of ab50339 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50339, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Huang G  et al. Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway. Int J Mol Med 39:559-568 (2017). Human . Read more (PubMed: 28112381) »
  • Guo H  et al. Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKa-regulated autophagy induction in streptozotocin-induced diabetic nephropathy. Sci Rep 7:6852 (2017). Read more (PubMed: 28761152) »

See all 24 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Kidney)
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Specification
Kidney
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Ms. Jeremy Song

Verified customer

提交于 May 16 2012

Thank you for your reply.
I have issued you the testing discount codes for ab82108 and ab82107 for testing in rat and pig. Please see below for the information.
For ab50339 I am sending you a testing discount code for testing in IHC-P. Since ...

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Thank you for your email.
I am sorry to hear that these antibodies are not working as expected.
As for ab58968, we had replaced this antibody free of charge with ab85379. Since ab85379 was not tested in rat, sheep, and pig tissue, I had sent ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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