Anti-NPFF1 Receptor抗体(ab45350)


  • 产品名称Anti-NPFF1 Receptor抗体
    参阅全部 NPFF1 Receptor 一抗
  • 描述
    兔多克隆抗体to NPFF1 Receptor
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from within residues 350 to the C-terminus of Human NPFF1 Receptor.

    (Peptide available as ab45349.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: SHSY5Y.

      This antibody gave a positive signal in the following cell lines: PC12.



Our Abpromise guarantee covers the use of ab45350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human NPFF1 Receptor peptide (ab45349).
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G Protein Coupled Receptor 147 antibody
    • G-protein coupled receptor 147 antibody
    • GPR147 antibody
    • Neuropeptide FF receptor 1 antibody
    • NPFF1 antibody
    • NPFF1_HUMAN antibody
    • NPFF1R1 antibody
    • NPFFR1 antibody
    • RFamide related peptide receptor OT7T022 antibody
    • RFamide-related peptide receptor OT7T022 antibody
    see all

Anti-NPFF1 Receptor antibody 图像

  • All lanes : Anti-NPFF1 Receptor antibody (ab45350) at 2 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Human NPFF1 Receptor peptide (ab45349) at 1 µg/ml

    Lysates/proteins at 15 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab45350 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NPFF1 Receptor antibody (ab45350)参考文献

ab45350 has not yet been referenced specifically in any publications.

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DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab45350 inICC/IF on mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abr...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No

Dr. Sophie Pezet

Verified customer

提交于 Oct 08 2008