Anti-NPAS2抗体(ab55833)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to NPAS2
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Synthetic peptide corresponding to N-terminal residues of human NPAS2.

性能

应用

Our Abpromise guarantee covers the use of ab55833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    BMAL1-NPAS2 heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.
  • 序列相似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic helix loop helix PAS protein MOP4 antibody
    • Basic-helix-loop-helix-PAS protein MOP4 antibody
    • bHLHe9 antibody
    • class E basic helix loop helix protein 9 antibody
    • Class E basic helix-loop-helix protein 9 antibody
    • FLJ23138 antibody
    • Member of PAS protein 4 antibody
    • Member of PAS superfamily 4 antibody
    • MGC71151 antibody
    • MOP4 antibody
    • Neuronal PAS domain containing protein 2 antibody
    • Neuronal PAS domain protein 2 antibody
    • Neuronal PAS domain-containing protein 2 antibody
    • Neuronal PAS2 antibody
    • NPAS2 antibody
    • NPAS2_HUMAN antibody
    • PAS domain containing protein 4 antibody
    • PAS domain-containing protein 4 antibody
    • PASD4 antibody
    see all

图片

  • Anti-NPAS2 antibody (ab55833) at 1/500 dilution + Mouse brain whole tissue lysate at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 92 kDa


    Exposure time : 20 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

实验方案

文献

ab55833 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting Abcam.

The immunogen sequence for ab55833 is ********************************.

Please let me know if there is anything else I can help you with.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
25 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册