概述

  • 产品名称Anti-Noxa抗体
    参阅全部 Noxa 一抗
  • 描述
    兔多克隆抗体to Noxa
  • 特异性ab23563 recognises a 16.5kDa Noxa molecule. Western Blot analysis of cells infected with Noxa adenoviruses and BAF indicates a high level of specificity for this antiserum,
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide:

    CAQLRRIGDKVNLRQK

    , corresponding to C terminal amino acids 75/90 of Mouse Noxa, coupled to Diphtheria Toxoid via a cysteine molecule added at the amino terminal of the peptide sequence.

性能

应用

Our Abpromise guarantee covers the use of ab23563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 16 kDa.

靶标

  • 功能Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
  • 组织特异性Highly expressed in adult T-cell leukemia cell line.
  • 序列相似性Belongs to the PMAIP1 family.
  • 结构域The BH3 motif is essential for pro-apoptotic activity.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adult T cell leukemia derived PMA responsive antibody
    • APR antibody
    • APR_HUMAN antibody
    • ATL-derived antibody
    • Immediate early response protein APR antibody
    • Immediate-early-response protein APR antibody
    • NOXA antibody
    • Phorbol 12 myristate 13 acetate induced protein 1 antibody
    • Phorbol-12-myristate-13-acetate-induced protein 1 antibody
    • PMA induced protein 1 antibody
    • PMA-induced protein 1 antibody
    • PMA-responsive gene antibody
    • Pmaip1 antibody
    • Protein Noxa antibody
    see all

Anti-Noxa antibody (ab23563)参考文献

This product has been referenced in:
  • Pfeiffer S  et al. Analysis of BH3-only proteins upregulated in response to oxygen/glucose deprivation in cortical neurons identifies Bmf but not Noxa as potential mediator of neuronal injury. Cell Death Dis 5:e1456 (2014). WB ; Mouse . Read more (PubMed: 25299781) »
  • Lainey E  et al. Azacytidine and erlotinib exert synergistic effects against acute myeloid leukemia. Oncogene : (2012). Read more (PubMed: 23085751) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cultured Cells (Primary cortical neurons)
Permeabilization Yes - 0,1% Triton in PBS
Specification Primary cortical neurons
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Dr. Cristina Muñoz-Pinedo

Verified customer

提交于 Feb 02 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Primary mouse neurons)
Permeabilization Yes - Triton 0.2%
Gating Strategy Live and dead cells
Specification Primary mouse neurons
Preparation Cell harvesting/tissue preparation method: (1200rpm, 5' at 4ºC) serial centrifugation
Sample buffer: PBS-BSA 1%
Fixation Paraformaldehyde
Username

Dr. Cristina Muñoz-Pinedo

Verified customer

提交于 Feb 02 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (14)
Sample Mouse Cell lysate - whole cell (Primary neurons as well as spleen/brain tissue)
Specification Primary neurons as well as spleen/brain tissue
Treatment Oxygen/glucose deprivation
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Cristina Muñoz-Pinedo

Verified customer

提交于 Feb 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"