Anti-Noxa抗体[114C307] (ab13654)

概述

  • 产品名称
    Anti-Noxa抗体[114C307]
    参阅全部 Noxa 一抗
  • 描述
    小鼠单克隆抗体[114C307] to Noxa
  • 经测试应用
    适用于: Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Fusion protein containing human Noxa.

性能

应用

Our Abpromise guarantee covers the use of ab13654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 11 kDa.

靶标

  • 功能
    Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
  • 组织特异性
    Highly expressed in adult T-cell leukemia cell line.
  • 序列相似性
    Belongs to the PMAIP1 family.
  • 结构域
    The BH3 motif is essential for pro-apoptotic activity.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adult T cell leukemia derived PMA responsive antibody
    • APR antibody
    • APR_HUMAN antibody
    • ATL-derived antibody
    • Immediate early response protein APR antibody
    • Immediate-early-response protein APR antibody
    • NOXA antibody
    • Phorbol 12 myristate 13 acetate induced protein 1 antibody
    • Phorbol-12-myristate-13-acetate-induced protein 1 antibody
    • PMA induced protein 1 antibody
    • PMA-induced protein 1 antibody
    • PMA-responsive gene antibody
    • Pmaip1 antibody
    • Protein Noxa antibody
    see all

图片

  • Western blot detection of Noxa in RL-7 cell (a follicular lymphoma) lysate. A protein of approximate molecular weight of 11 kDa is detected using ab13654 at 2 µg/ml.

    Western blot detection of Noxa in RL-7 cell (a follicular lymphoma) lysate. A protein of approximate molecular weight of 11 kDa is detected using ab13654 at 2 µg/ml.
  • Overlay histogram showing Jurkat cells stained with ab13654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13654, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

文献

This product has been referenced in:
  • Yokoyama T  et al. Apoptosis is augmented in high-grade serous ovarian cancer by the combined inhibition of Bcl-2/Bcl-xL and PARP. Int J Oncol N/A:N/A (2017). WB ; Human . Read more (PubMed: 28350129) »
  • Inoue-Yamauchi A  et al. Targeting the differential addiction to anti-apoptotic BCL-2 family for cancer therapy. Nat Commun 8:16078 (2017). Read more (PubMed: 28714472) »

See all 36 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (18% gel)
Sample
Human Cell lysate - whole cell (Breast)
Specification
Breast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Jan 02 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your enquiry regarding ab13654. Noxa is highly expressed in adult T-cell leukemia cell line. Since Jurkat cells are T cell leukemia cell line, it is a good positive control for studying Noxa. Under the References section of Swiss-Pr...

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Thank you for contacting us.  I agree that the antibody is not working as expected, and I would be happy to offer you a free of charge replacement, credit, or refund if you have purchased the antibody in the past six months.  It would be very helpful f...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Myeloma)
Loading amount
30 µg
Specification
Myeloma
Treatment
1.25 uM RITA for 6 hrs
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Manujendra

Verified customer

提交于 Jan 07 2011

Thank you for your enquiry. Here are some comments and suggestions: 1. You mention using a high dilution of the secondary antibody? What dilution did you use? 2. Further dilution of the primary antibody, perhaps to 0.5ug/ml or 0.25ug/ml, will ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Loading amount
20 µg
Specification
HL60
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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