Anti-NOX3抗体(ab81864)

概述

  • 产品名称Anti-NOX3抗体
    参阅全部 NOX3 一抗
  • 描述
    兔多克隆抗体to NOX3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NOX3.

    (Peptide available as ab101218.)

  • 阳性对照
    • This antibody gave a positive signal in HeLa and HEK293 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab81864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

Anti-NOX3 antibody 图像

  • All lanes : Anti-NOX3 antibody (ab81864) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab81864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% MCF7 fixed (10 min) MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml

Anti-NOX3 antibody (ab81864)参考文献

ab81864 has not yet been referenced specifically in any publications.

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