Anti-NOX2/gp91phox抗体(ab80508)

概述

  • 产品名称Anti-NOX2/gp91phox抗体
    参阅全部 NOX2/gp91phox 一抗
  • 描述
    兔多克隆抗体to NOX2/gp91phox
  • 经测试应用适用于: ICC/IF, Flow Cyt, IHC-Fr, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Cow, Dog
  • 免疫原

    Synthetic peptide: KTIASQHPNT RIGVFLCGPE ALAETLSKQS ISNSESGPRG VHFIFNKENF , corresponding to C terminal amino acids 522-571 of Human NOX2/gp91phox (NP_000388) (Peptides available as ab111671).

  • 阳性对照
    • MCF7 cell lysate. THP-1 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).Can be blocked with Human NOX2/gp91phox peptide (ab111671). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
  • 疾病相关Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • 序列相似性Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • 翻译后修饰Glycosylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMCBX2 antibody
    • CGD antibody
    • CGD91-phox antibody
    • CY24B_HUMAN antibody
    • CYBB antibody
    • Cytochrome b 245, beta polypeptide antibody
    • Cytochrome b(558) beta chain antibody
    • Cytochrome b(558) subunit beta antibody
    • Cytochrome b-245 heavy chain antibody
    • Cytochrome b558 subunit beta antibody
    • GP91 PHOX antibody
    • gp91-1 antibody
    • gp91-phox antibody
    • GP91PHOX antibody
    • Heme-binding membrane glycoprotein gp91phox antibody
    • NADPH oxidase 2 antibody
    • Neutrophil cytochrome b 91 kDa polypeptide antibody
    • NOX2 antibody
    • p22 phagocyte B-cytochrome antibody
    • P91 PHOX antibody
    • p91-PHOX antibody
    • Superoxide-generating NADPH oxidase heavy chain subunit antibody
    see all

Anti-NOX2/gp91phox antibody 图像

  • Anti-NOX2/gp91phox antibody (ab80508) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
  • ab80508 staining NOX2/gp91phox in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% milk for 30 minutes at 37°C; antigen retrieval was by heat mediation with a Tris Borate EDTA buffer (pH 8.4). Samples were incubated with primary antibody (1/200 in antibody diluent) for 1 hour at 37°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/700) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab80508 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80508, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab80508 staining NOX2/gp91phox in Human monocyte-derivive dendritic cells by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with methanol. The sample was incubated with the primary antibody (1/200) for 30 minutes. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/300) was used as the secondary antibody

    See Abreview

  • All lanes : Anti-NOX2/gp91phox antibody (ab80508) at 1/1000 dilution

    Lane 1 : Mouse liver whole cell lysate
    Lane 2 : Mouse liver whole cell lysate

    Lysates/proteins at 100 µg per lane.

    Secondary
    HRP-conjugated Goat polyclonal to rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 65 kDa


    Exposure time : 5 seconds

    This image is a courtesy of an anonymous abreview.

    Primary incubated for 9 hours at 4°C.

    See Abreview

Anti-NOX2/gp91phox antibody (ab80508)参考文献

This product has been referenced in:
  • Lim R  et al. Activin and NADPH-oxidase in preeclampsia: insights from in vitro and murine studies. Am J Obstet Gynecol 212:86.e1-12 (2015). Read more (PubMed: 25046804) »
  • Koba S  et al. Oxidative stress exaggerates skeletal muscle contraction-evoked reflex sympathoexcitation in rats with hypertension induced by angiotensin II. Am J Physiol Heart Circ Physiol 304:H142-53 (2013). WB . Read more (PubMed: 23086992) »

See all 11 Publications for this product

Product Wall

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. Thank you for ...

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Application Western blot
Sample Rat Tissue lysate - whole (muscle)
Gel Running Conditions Reduced Denaturing (9%)
Loading amount 60 µg
Specification muscle
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Baptiste Jude

Verified customer

提交于 Jul 18 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2016

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have following further questions for better understanding of the problem;

Lysis buffer
Fresh Protease inhibitors: yes/no<...

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While we do not have specific data regarding the possible cross reactivity of this item with other Nox proteins, we are confident of the specificity of this item based on sequence homology of the immunogen against Nox1, 3, 4, 5. Generally we find that ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Loading amount 50 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 Sep 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Single isolated FDB skeletal muscle fibres)
Specification Single isolated FDB skeletal muscle fibres
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 (for 10 hours)
Blocking step BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 Sep 25 2012

Thank you for contacting us yesterday in regards to the anti-NOX2/gp91phox antibody (http://www.abcam.com/NOX2-gp91phox-antibody-ab80508.html) which is currently unfortunately out of stock.

As discussed over the phone, I have performed a sequ...

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I have raised a free of charge order for the blocking peptide used as immunogen for ab80508.
The order number is XXXXX. You should receive an automatic confirmation.
I hope this would help you to identify the correct target band.
I wish ...

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