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Synthetic peptide: KTIASQHPNT RIGVFLCGPE ALAETLSKQS ISNSESGPRG VHFIFNKENF , corresponding to C terminal amino acids 522-571 of Human NOX2/gp91phox (NP_000388) (Peptides available as ab111671).
Our Abpromise guarantee covers the use of ab80508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).Can be blocked with Human NOX2/gp91phox peptide (ab111671). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
This image is a courtesy of an anonymous abreview.
Primary incubated for 9 hours at 4°C.
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