概述

  • 产品名称Anti-NOX1抗体
    参阅全部 NOX1 一抗
  • 描述
    兔多克隆抗体to NOX1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the C terminal of Human NOX1

  • 阳性对照
    • Human colon tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab78016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 65 kDa).
ELISA Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
  • 组织特异性NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells.
  • 序列相似性Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • 细胞定位Cell projection > invadopodium membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GP91 2 antibody
    • Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
    • Mitogenic oxidase 1 antibody
    • MOX-1 antibody
    • MOX1 antibody
    • NADH/NADPH mitogenic oxidase subunit P65 MOX antibody
    • NADH/NADPH mitogenic oxidase subunit P65-MOX antibody
    • NADPH oxidase 1 antibody
    • NADPH oxidase 1 variant NOH 1L antibody
    • NADPH oxidase homolog 1 antibody
    • NOH 1 antibody
    • NOH-1 antibody
    • NOH1 antibody
    • NOX-1 antibody
    • Nox1 antibody
    • NOX1_HUMAN antibody
    • RP1 146H21.1 antibody
    see all

Anti-NOX1 antibody 图像

  • ab78016, at 10µg/ml, staining NOX1 in Human colon by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • Anti-NOX1 antibody (ab78016) at 1 µg/ml + Human Prostate Tumor Lysate Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-NOX1 antibody (ab78016)参考文献

This product has been referenced in:
  • Kim KA  et al. NOX1 participates in ROS-dependent cell death of colon epithelial Caco2 cells induced by Entamoeba histolytica. Microbes Infect 13:1052-61 (2011). WB ; Human . Read more (PubMed: 21723410) »
  • Shimada K  et al. A novel human AlkB homologue, ALKBH8, contributes to human bladder cancer progression. Cancer Res 69:3157-64 (2009). WB, ICC ; Human . Read more (PubMed: 19293182) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human mast cell)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 10 µg
Specification Human mast cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Seong Hoon Kim

Verified customer

提交于 Jun 23 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (colon cancer cells)
Specification colon cancer cells
Treatment pathogen (Entamoeba histolytica)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 27 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"