概述

  • 产品名称
  • 描述
    山羊多克隆抗体to NOX1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide:

    DKATDIVTGLKQK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 490-502 of Human NOX1 (NP_008983.2) or 441-453 of Human NOX1 (NP_039249.1).

  • 阳性对照
    • Human and Mouse colon lysates, cultured transgenic Mouse aortic smooth muscle cells (MASM).

性能

应用

Our Abpromise guarantee covers the use of ab121009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

靶标

  • 功能
    NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
  • 组织特异性
    NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells.
  • 序列相似性
    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • 细胞定位
    Cell projection > invadopodium membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GP91 2 antibody
    • Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
    • Mitogenic oxidase 1 antibody
    • MOX-1 antibody
    • MOX1 antibody
    • NADH/NADPH mitogenic oxidase subunit P65 MOX antibody
    • NADH/NADPH mitogenic oxidase subunit P65-MOX antibody
    • NADPH oxidase 1 antibody
    • NADPH oxidase 1 variant NOH 1L antibody
    • NADPH oxidase homolog 1 antibody
    • NOH 1 antibody
    • NOH-1 antibody
    • NOH1 antibody
    • NOX-1 antibody
    • Nox1 antibody
    • NOX1_HUMAN antibody
    • RP1 146H21.1 antibody
    see all

图片

  • All lanes : Anti-NOX1 antibody (ab121009) at 0.1 µg/ml

    Lane 1 : Colorectal adenocarcinoma cell lysate (35µg protein in RIPA buffer)
    Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer)


    Predicted band size : 65 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • All lanes : Anti-NOX1 antibody (ab121009) at 1 µg/ml

    Lane 1 : WT MASM cell lysate (in RIPA buffer)
    Lane 2 : Knock out MASM cell lysate (in RIPA buffer)
    Lane 3 : Transgenic MASM cell lysate (in RIPA buffer)
    Lane 4 : Mouse colon lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 65 kDa

实验方案

文献

ab121009 has not yet been referenced specifically in any publications.

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