Mouse, Rat, Dog, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Nova1.
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; MCF7; A431.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
Anti-Nova1 antibody 图像
Western blot - Nova1 antibody (ab94511)
All lanes :
Anti-Nova1 antibody (ab94511) at 1 µg/mlLane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 :
MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 :
A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.Predicted band size :
48 kDaObserved band size :
50 kDa (why is the actual band size different from the predicted?
)Additional bands at :
15 kDa,27 kDa. We are unsure as to the identity of these extra bands.Exposure time :
Anti-Nova1 antibody (ab94511)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"