Mouse, Rat, Dog, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Nova1.
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; MCF7; A431.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
May regulate RNA splicing or metabolism in a specific subset of developing neurons.
Contains 3 KH domains.
Information by UniProt
- Neuro oncological ventral antigen 1 antibody
- Neuro-oncological ventral antigen 1 antibody
- NOVA 1 antibody
Western blot - Anti-Nova1 antibody (ab94511)
All lanes :
Anti-Nova1 antibody (ab94511) at 1 µg/mlLane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 :
MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 :
A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
48 kDaObserved band size:
50 kDa (why is the actual band size different from the predicted?
)Additional bands at:
15 kDa, 27 kDa. We are unsure as to the identity of these extra bands.Exposure time:
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"