ab77594 is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1).
Mouse, Rat, Cynomolgus monkey
, corresponding to internal sequence amino acids 124-137 of Human Nova1 (NP_002506.2 ; NP_006480.2 ; NP_006482.1).
Human breast cancer lysate. Transfected HEK293 transiently expressing Nova1. Human cerebral cortex tissue sections.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
ab77594 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Use a concentration of 2 - 4 µg/ml.
May regulate RNA splicing or metabolism in a specific subset of developing neurons.
Contains 3 KH domains.
Information by UniProt
Neuro oncological ventral antigen 1 antibody
Neuro-oncological ventral antigen 1 antibody
NOVA 1 antibody
Western blot - Anti-Nova1 antibody (ab77594)
Anti-Nova1 antibody (ab77594) at 0.01 µg/ml + Human Breast Cancer lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique. Predicted band size: 52 kDa Observed band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody (ab77594)
ab77594 at 2.5µg/ml staining Nova1 in human cerebral cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval was performed with citrate buffer pH 6, AP-staining.
Western blot - Nova1 antibody (ab77594)
All lanes : Anti-Nova1 antibody (ab77594) at 0.03 µg/ml Lane 1 : Lysate prepared from MOCK transfection. Lane 2 : Lysate prepared from transfected HEK293 cells, transiently expressing Nova1 Predicted band size: 52 kDa
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