概述

  • 产品名称Anti-Nova1抗体
    参阅全部 Nova1 一抗
  • 描述
    山羊多克隆抗体to Nova1
  • 特异性ab77594 is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1).
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    REMPQNVAKTEPVS

    , corresponding to internal sequence amino acids 124-137 of Human Nova1 (NP_002506.2 ; NP_006480.2 ; NP_006482.1).

  • 阳性对照
    • Human breast cancer lysate. Transfected HEK293 transiently expressing Nova1. Human cerebral cortex tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab77594 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.

靶标

Anti-Nova1 antibody 图像

  • Anti-Nova1 antibody (ab77594) at 0.01 µg/ml + Human Breast Cancer lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab77594 at 2.5µg/ml staining Nova1 in human cerebral cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval was performed with citrate buffer pH 6, AP-staining.
  • All lanes : Anti-Nova1 antibody (ab77594) at 0.03 µg/ml

    Lane 1 : Lysate prepared from MOCK transfection.
    Lane 2 : Lysate prepared from transfected HEK293 cells, transiently expressing Nova1


    Predicted band size : 52 kDa

Anti-Nova1 antibody (ab77594)参考文献

This product has been referenced in:
  • Zhang YA  et al. High expression of neuro-oncological ventral antigen 1 correlates with poor prognosis in hepatocellular carcinoma. PLoS One 9:e90955 (2014). Human . Read more (PubMed: 24608171) »
  • Morchikh M  et al. TOX4 and NOVA1 Proteins Are Partners of the LEDGF PWWP Domain and Affect HIV-1 Replication. PLoS One 8:e81217 (2013). WB ; Human . Read more (PubMed: 24312278) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (Any kD (Bio-Rad))
Sample Mouse Tissue lysate - whole (whole brain)
Specification whole brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Mar 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"