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DHFHNGHCEKGCNN, corresponding to internal sequence amino acids 1261-1273 of Human NOTCH4 (NP_004548.3). The immunizing peptide represents part of the extracellular domain.
|WB||Use at an assay dependent concentration. Preliminary experiments gave an approx 27kDa band in all Human, Mouse and Rat tissue and cell line lysates tested after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 210kDa according to NP_004548.3. The 27kDa band was successfully blocked by incubation with the immunizing peptide.|
ab121004 has not yet been referenced specifically in any publications.