概述

  • 产品名称Anti-NOTCH3抗体
    参阅全部 NOTCH3 一抗
  • 描述
    兔多克隆抗体to NOTCH3
  • 经测试应用适用于: IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide representing the C terminal region of Human NOTCH3.

  • 阳性对照
    • Rat cerebellum

性能

应用

Our Abpromise guarantee covers the use of ab92758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
  • 应用说明IHC-Fr: 1/100 - 1/300.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
    • 组织特异性Ubiquitously expressed in fetal and adult tissues.
    • 疾病相关Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [MIM:125310]. CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.
    • 序列相似性Belongs to the NOTCH family.
      Contains 5 ANK repeats.
      Contains 34 EGF-like domains.
      Contains 3 LNR (Lin/Notch) repeats.
    • 翻译后修饰Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
      Phosphorylated.
    • 细胞定位Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CADASIL antibody
      • CASIL antibody
      • NOTC3_HUMAN antibody
      • Notch 3 antibody
      • Notch 3 intracellular domain antibody
      • Notch homolog 3 antibody
      • Notch3 antibody
      see all

    Anti-NOTCH3 antibody 图像

    • ab92758 at 1/100 dilution staining NOTCH3 in (Bergmann glia-10 micron cryostat sections) rat cerebellum by Immunohistochemistry.

    Anti-NOTCH3 antibody (ab92758)参考文献

    ab92758 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92758.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"