Anti-NOTCH3抗体(ab60087)

概述

  • 产品名称Anti-NOTCH3抗体
    参阅全部 NOTCH3 一抗
  • 描述
    兔多克隆抗体to NOTCH3
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human NOTCH3.

  • 阳性对照
    • Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab60087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
  • 组织特异性Ubiquitously expressed in fetal and adult tissues.
  • 疾病相关Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [MIM:125310]. CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.
  • 序列相似性Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 34 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • 翻译后修饰Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
  • 细胞定位Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CADASIL antibody
    • CASIL antibody
    • NOTC3_HUMAN antibody
    • Notch 3 antibody
    • Notch 3 intracellular domain antibody
    • Notch homolog 3 antibody
    • Notch3 antibody
    see all

Anti-NOTCH3 antibody 图像

  • Ab60087 (1/50-1/100) staining human NOTCH3 in human cancer tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    BC = breast carcinoma.
  • ICC/IF image of ab60087 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60087, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NOTCH3 antibody (ab60087)参考文献

ab60087 has not yet been referenced specifically in any publications.

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab98765 and one vial of ab102409. To check the status of the order pl...

Read More

Thank you for contacting us. The MSDS can be downloaded online form our website. The link is given on every antibody datasheet in storage buffer section. Please also find attached with this email. Ab3856 does not contain any prese...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"