Anti-Notch2抗体(ab8926)
Key features and details
- Rabbit polyclonal to Notch2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Notch2抗体
参阅全部 Notch2 一抗 -
描述
兔多克隆抗体to Notch2 -
宿主
Rabbit -
特异性
The Notch2 protein has many isoforms. The immunogenic sequence for the anti-Notch2 antibody is identical to the same region in full length (260+ kDa) as well as the cleaved forms ~82 kDa each (Notch 2 extracellular truncation & Notch 2 intracellular domain) and the post translationally modified forms thereof.
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经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Notch2 aa 1700-1800 (N terminal) (Cysteine residue). The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Database link: Q04721 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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IHC-P |
1/500.
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说明 |
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IHC-P
1/500. |
靶标
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功能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737). -
组织特异性
Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo. -
疾病相关
Alagille syndrome 2
Hajdu-Cheney syndrome -
序列相似性
Belongs to the NOTCH family.
Contains 6 ANK repeats.
Contains 35 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats. -
翻译后修饰
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Hydroxylated by HIF1AN.
Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1. -
细胞定位
Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737). - Information by UniProt
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数据库链接
- Entrez Gene: 4853 Human
- Entrez Gene: 18129 Mouse
- Omim: 600275 Human
- SwissProt: Q04721 Human
- SwissProt: O35516 Mouse
- SwissProt: Q9QW30 Rat
- Unigene: 487360 Human
- Unigene: 485843 Mouse
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别名
- AGS2 antibody
- hN2 antibody
- Motch B antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is expressed in germ cells entering meiosis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is ubiquitously expressed in germ cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Notch2 with ab8926 at 1/500 dilution. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (63)
ab8926 被引用在 63 文献中.
- Chen LJ et al. Gm364 coordinates MIB2/DLL3/Notch2 to regulate female fertility through AKT activation. Cell Death Differ 29:366-380 (2022). PubMed: 34635817
- Zhang S et al. GIT1 protects against breast cancer growth through negative regulation of Notch. Nat Commun 13:1537 (2022). PubMed: 35318302
- Silva E et al. Immunolocalization of Notch Signaling in Mouse Preimplantation Embryos. Methods Mol Biol 2472:173-185 (2022). PubMed: 35674900
- Koba H et al. NOTCH alteration in EGFR-mutated lung adenocarcinoma leads to histological small-cell carcinoma transformation under EGFR-TKI treatment. Transl Lung Cancer Res 10:4161-4173 (2021). PubMed: 35004247
- Martos-Rodríguez CJ et al. Fibrous Caps in Atherosclerosis Form by Notch-Dependent Mechanisms Common to Arterial Media Development. Arterioscler Thromb Vasc Biol 41:e427-e439 (2021). PubMed: 34261328