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Synthetic peptide corresponding to a region within c-terminal amino acids 2506-2555 (EHPFLTPSPE SPDQWSSSSP HSNVSDWSEG VSSPPTSMQS QIARIPEAFK) of Human Notch1 intracellular domain (NP_060087).
Our Abpromise guarantee covers the use of ab83232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 273 kDa for Notch1 full length protein and 88 kDa for Notch1 intracellular domain. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-Fr||Use at an assay dependent concentration.|
ab83232 has not yet been referenced specifically in any publications.