Anti-Notch1抗体[4i50] (ab64570)


  • 产品名称Anti-Notch1抗体[4i50]
    参阅全部 Notch1 一抗
  • 描述
    叙利亚仓鼠单克隆抗体[4i50] to Notch1
  • 经测试应用适用于: WB, ELISA, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    GST fusion protein corresponding to residues 1299-1492 of mouse Notch1.

  • 阳性对照
    • mouse C2C12 muscle myoblast cell lysates.



Our Abpromise guarantee covers the use of ab64570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明ELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    WB: 1/250 - 1/500. Detects a band of approximately 300 kDa (predicted molecular weight: 270 kDa). Additional proteins were detected at 230 kDa and 160 kDa, believed to be the processed extracellular fragments of Notch 1.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
    • 组织特异性In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
    • 疾病相关Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
    • 序列相似性Belongs to the NOTCH family.
      Contains 5 ANK repeats.
      Contains 36 EGF-like domains.
      Contains 3 LNR (Lin/Notch) repeats.
    • 翻译后修饰Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
      O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
      Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
    • 细胞定位Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 9930111A19Rik antibody
      • AOS5 antibody
      • AOVD1 antibody
      • hN1 antibody
      • Lin-12 antibody
      • LIN12 antibody
      • Mis6 antibody
      • Motch A antibody
      • mT14 antibody
      • Neurogenic locus notch homolog protein 1 antibody
      • Neurogenic locus notch protein homolog antibody
      • NICD antibody
      • NOTC1_HUMAN antibody
      • Notch 1 antibody
      • Notch 1 intracellular domain antibody
      • NOTCH antibody
      • Notch gene homolog 1 (Drosophila) antibody
      • Notch homolog 1, translocation-associated (Drosophila) antibody
      • NOTCH, Drosophila, homolog of, 1 antibody
      • notch1 antibody
      • TAN1 antibody
      • Translocation associated notch homolog antibody
      • Translocation associated notch protein TAN 1 antibody
      • Translocation-associated notch protein TAN-1 antibody
      • xotch antibody
      see all

    Anti-Notch1 antibody [4i50] (ab64570)参考文献

    ab64570 has not yet been referenced specifically in any publications.

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