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Synthetic peptide corresponding to N terminal residues of Norovirus Capsid protein VP1
Our Abpromise guarantee covers the use of ab92976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ELISA||1/2000 - 1/5000.|
Ab92976 staining Norovirus Capsid protein VP1 in RAW 264.7 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Digitonin (50µg/ml) and blocked with 1% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 dilution) for 1 hour at 21°C. An Alexa Fluor® 488 Donkey Anti-Mouse was used as the secondary antibody at 1/1000 dilution.