Anti-Norovirus Capsid protein VP1抗体(ab92976)

概述

  • 产品名称Anti-Norovirus Capsid protein VP1抗体
  • 描述
    兔多克隆抗体to Norovirus Capsid protein VP1
  • 特异性Species Reactivity: Norovirus Hu/Texas/TCH04-577/2004/US
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Other
  • 免疫原

    Synthetic peptide corresponding to N terminal residues of Norovirus Capsid protein VP1

性能

应用

Our Abpromise guarantee covers the use of ab92976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/2000 - 1/5000.

靶标

  • 相关性Norovirus is an RNA virus of the Caliciviridae taxonomic family, causes approximately 90% of epidemic non bacterial outbreaks of gastroenteritis around the world and is responsible for 50% of all foodborne outbreaks of gastroenteritis in the US. Norovirus affects people of all ages. The viruses are transmitted by faecally contaminated food or water and by person to person contact. Noroviruses contain a positive-sense RNA genome of approximately 7.5 kbp, encoding a major structural protein (VP1) of about 58~60 kDa and a minor capsid protein (VP2). The virus particles demonstrate an amorphous surface structure when visualized using electron microscopy and are between 27-38 nm in size. Capsid protein VP1 attaches virion to target cells by binding histo-blood group antigens present on gastroduodenal epithelial cells. Soluble capsid protein may play a role in viral immunoevasion. Capsid protein VP1 binds to histoblood group antigens at surface of target cells. The shell domain (S domain) contains elements essential for the formation of the icosahedron. The Protruding domain (P domain) is divided into subdomains P1 and P2. P domain interacts in dimeric contacts that increase the stability of the capsid and form the protrusions on the virion. An hypervariable region in P2 is thought to play an important role in receptor binding and immune reactivity.
  • 别名
    • VP1 antibody

Anti-Norovirus Capsid protein VP1 antibody (ab92976)参考文献

This product has been referenced in:
  • Hong SA  et al. A rapid, sensitive and selective electrochemical biosensor with concanavalin A for the preemptive detection of norovirus. Biosens Bioelectron 64C:338-344 (2014). Read more (PubMed: 25254625) »
  • Jones MK  et al. Enteric bacteria promote human and mouse norovirus infection of B cells. Science 346:755-9 (2014). Neutralising . Read more (PubMed: 25378626) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Mouse Cell lysate - whole cell (macrophage cell line)
Specification macrophage cell line
Treatment MNV infection
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

提交于 Oct 29 2014

Thank you for contacting us.

The antibodies have only been tested in ELISA. We do not have any data for western blotting.

Several of these are monoclonal antibodies that are raised against various strains of Norovirus, and the epi...

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Thank you for your inquiry.
I translated your sequence into the following protein sequence:
MRMSDGAAPKANGSEASGQDLVPTAVEQAVPIQPVAGAALAAPAAGQINQIDPWIFQNFVQCPLGEFSISPRNTPGEILFDLALGPGLNPYLAHLSAMYTGWVGNMEVQLVLAGNAFTAGKVVVALVPPYFPKGSLTTAQITCFPHVMCD...

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Thank you for your reply. There is about a 74% similarity between your sequence the immunogen sequence that was used for ab92976. Normally we look for about an 85% similarity before recommending an antibody be used on an untested species. If you will s...

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Unfortunately I cannot determine if the antibody will react with the strain that you are working on. If you could send me the sequence of your particular strain then I could determine if there is a significant match between that and the peptide sequenc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"